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Literature summary extracted from

  • Hendren, R.W.; Bloch, K.
    Fatty acid synthetases from Euglena gracilis. Separation of component activities of the ACP-dependent fatty acid synthetase and partial purification of the beta-ketoacyl-ACP synthetase (1980), J. Biol. Chem., 255, 1504-1508.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.41 freezing and thawing inactivates Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.41 chloroplast
-
 Euglena gracilis 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 44000
-
 gel filtration Euglena gracilis
2.3.1.38 147000
-
 gel filtration Euglena gracilis
2.3.1.41 118000
-
 gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Euglena gracilis enzyme is component of fatty acid synthase complex II CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Euglena gracilis Z enzyme is component of fatty acid synthase complex II CoA + acetyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Euglena gracilis
-
-
-
2.3.1.38 Euglena gracilis
-
 Z
-
2.3.1.38 Euglena gracilis Z
-
 Z
-
2.3.1.39 Euglena gracilis
-
 Z
-
2.3.1.39 Euglena gracilis Z
-
 Z
-
2.3.1.41 Euglena gracilis
-
 Z
-
2.3.1.41 Euglena gracilis Z
-
 Z
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 partial Euglena gracilis
2.3.1.41
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.38 additional information
-
 fatty acid synthase II assay Euglena gracilis
2.3.1.41 3.22
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Euglena gracilis (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Euglena gracilis Z (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Euglena gracilis CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] enzyme is component of fatty acid synthase complex II Euglena gracilis CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Euglena gracilis Z CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] enzyme is component of fatty acid synthase complex II Euglena gracilis Z CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Euglena gracilis CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Euglena gracilis Z CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Euglena gracilis 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Euglena gracilis Z 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.38 acetyl coenzyme A:ACP transacylase
-
 Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.38 25
-
 assay at Euglena gracilis
2.3.1.39 25
-
 assay at Euglena gracilis
2.3.1.41 35
-
 assay at Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.38 6.8
-
 assay at Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 additional information NADH is used by NADH-dependent 3-oxoacyl [ACP]-reductase, EC 1.1.1.121 Euglena gracilis
1.1.1.100 NADPH
-
 Euglena gracilis