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Literature summary extracted from

  • Shimakata, T.; Stumpf, P.K.
    The purification and function of acetyl coenzyme A:acyl carrier protein transacylase (1983), J. Biol. Chem., 258, 3592-3598.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.38 arsenite
-
 Spinacia oleracea
2.3.1.38 N-ethylmaleimide
-
 Spinacia oleracea
2.3.1.38 p-Substituted mercuribenzoate
-
 Spinacia oleracea
2.3.1.39 additional information
-
 Brassica juncea
2.3.1.39 additional information
-
 Carthamus tinctorius
2.3.1.39 additional information
-
 Cuphea lutea
2.3.1.39 additional information
-
 Pisum sativum
2.3.1.39 additional information arsenite Spinacia oleracea
2.3.1.41 arsenite
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.38 0.008
-
 acetyl-CoA
-
 Spinacia oleracea
2.3.1.38 0.0083
-
 butyryl-CoA
-
 Spinacia oleracea
2.3.1.38 0.0086
-
 hexanoyl-CoA
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.38 48000
-
 gel filtration Spinacia oleracea
2.3.1.41 56000
-
 synthase I, gel filtration Spinacia oleracea
2.3.1.41 57500
-
 synthase II, gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Carthamus tinctorius
-
 CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Spinacia oleracea increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] Spinacia oleracea rate-limiting step of fatty acid synthesis CoA + acetyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Brassica juncea
-
 rapeseed
-
1.1.1.100 Carthamus tinctorius
-
 safflower
-
1.1.1.100 Cuphea lutea
-
-
-
1.1.1.100 Persea americana
-
-
-
1.1.1.100 Pisum sativum
-
-
-
1.1.1.100 Spinacia oleracea
-
-
-
2.3.1.38 Brassica juncea
-
-
-
2.3.1.38 Carthamus tinctorius
-
 safflower
-
2.3.1.38 Cuphea lutea
-
-
-
2.3.1.38 Pisum sativum
-
-
-
2.3.1.38 Spinacia oleracea
-
-
-
2.3.1.39 Brassica juncea
-
 rape
-
2.3.1.39 Carthamus tinctorius
-
 safflower
-
2.3.1.39 Cuphea lutea
-
 Lythraceae
-
2.3.1.39 Pisum sativum
-
 pea
-
2.3.1.39 Spinacia oleracea
-
 spinach
-
2.3.1.41 Brassica juncea
-
-
-
2.3.1.41 Carthamus tinctorius
-
 safflower
-
2.3.1.41 Cuphea lutea
-
-
-
2.3.1.41 Pisum sativum
-
-
-
2.3.1.41 Spinacia oleracea
-
 synthase I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.38
-
 Spinacia oleracea
2.3.1.41 synthase I Spinacia oleracea
2.3.1.41 synthases II Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.100 leaf
-
 Spinacia oleracea
-
1.1.1.100 leaf
-
 Pisum sativum
-
1.1.1.100 seed
-
 Persea americana
-
1.1.1.100 seed
-
 Brassica juncea
-
1.1.1.100 seed
-
 Carthamus tinctorius
-
1.1.1.100 seed
-
 Cuphea lutea
-
2.3.1.38 leaf
-
 Spinacia oleracea
-
2.3.1.38 leaf
-
 Pisum sativum
-
2.3.1.38 seed
-
 Brassica juncea
-
2.3.1.38 seed
-
 Carthamus tinctorius
-
2.3.1.38 seed
-
 Cuphea lutea
-
2.3.1.39 seed
-
 Brassica juncea
-
2.3.1.39 seed
-
 Carthamus tinctorius
-
2.3.1.41 leaf
-
 Spinacia oleracea
-
2.3.1.41 leaf
-
 Pisum sativum
-
2.3.1.41 leaf
-
 Brassica juncea
-
2.3.1.41 leaf
-
 Carthamus tinctorius
-
2.3.1.41 leaf
-
 Cuphea lutea
-
2.3.1.41 seed germinating Spinacia oleracea
-
2.3.1.41 seed germinating Brassica juncea
-
2.3.1.41 seed germinating Carthamus tinctorius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 0.0132
-
-
 Pisum sativum
1.1.1.100 0.0168
-
-
 Cuphea lutea
1.1.1.100 0.0189
-
-
 Carthamus tinctorius
1.1.1.100 0.0192
-
-
 Spinacia oleracea
1.1.1.100 0.0366
-
-
 Brassica juncea
2.3.1.38 additional information
-
-
 Pisum sativum
2.3.1.38 additional information
-
-
 Brassica juncea
2.3.1.38 additional information
-
-
 Carthamus tinctorius
2.3.1.38 additional information
-
-
 Cuphea lutea
2.3.1.38 additional information
-
 fatty acid synthase II assay Spinacia oleracea
2.3.1.38 0.000005
-
-
 Pisum sativum
2.3.1.38 0.000009
-
 crude enzyme extract Spinacia oleracea
2.3.1.38 0.000018
-
-
 Carthamus tinctorius
2.3.1.38 0.000064
-
-
 Cuphea lutea
2.3.1.38 0.00019
-
-
 Brassica juncea
2.3.1.38 0.0056
-
 purified enzyme Spinacia oleracea
2.3.1.39 additional information
-
-
 Spinacia oleracea
2.3.1.39 additional information
-
-
 Pisum sativum
2.3.1.39 additional information
-
-
 Brassica juncea
2.3.1.39 additional information
-
-
 Carthamus tinctorius
2.3.1.39 additional information
-
-
 Cuphea lutea
2.3.1.41 additional information
-
-
 Spinacia oleracea
2.3.1.41 additional information
-
 fatty acid synthase activity, malonyl-CoA incorporation Pisum sativum
2.3.1.41 additional information
-
 fatty acid synthase activity, malonyl-CoA incorporation Carthamus tinctorius
2.3.1.41 additional information
-
 fatty acid synthase activity, malonyl-CoA incorporation Cuphea lutea

Storage Stability

EC Number Storage Stability Organism
2.3.1.38 -70°C, several months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Spinacia oleracea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Pisum sativum (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Persea americana (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Brassica juncea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Carthamus tinctorius (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Cuphea lutea (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Pisum sativum CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Brassica juncea CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Carthamus tinctorius CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein]
-
 Cuphea lutea CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] increase in enzyme level in reconsituted FAS system enhances production of medium length fatty acids Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 acetyl-CoA + [acyl-carrier protein] rate-limiting step of fatty acid synthesis Spinacia oleracea CoA + acetyl-[acyl-carrier protein]
-
 ?
2.3.1.38 butyryl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + butyryl-[acyl-carrier protein]
-
 ?
2.3.1.38 hexanoyl-CoA + [acyl-carrier protein]
-
 Spinacia oleracea CoA + hexanoyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Spinacia oleracea CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Pisum sativum CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Brassica juncea CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Carthamus tinctorius CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.39 malonyl-CoA + acyl-carrier protein via stable covalent malonyl-enzyme intermediate Cuphea lutea CoA + malonyl-[acyl-carrier protein]
-
 ?
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] preferred substrate, synthase III Spinacia oleracea 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Pisum sativum 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Brassica juncea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Carthamus tinctorius 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Cuphea lutea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.38 38
-
 assay at Spinacia oleracea
2.3.1.38 38
-
 assay at Pisum sativum
2.3.1.38 38
-
 assay at Brassica juncea
2.3.1.38 38
-
 assay at Carthamus tinctorius
2.3.1.38 38
-
 assay at Cuphea lutea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.38 8.1
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Spinacia oleracea
1.1.1.100 NADPH
-
 Pisum sativum
1.1.1.100 NADPH
-
 Persea americana
1.1.1.100 NADPH
-
 Brassica juncea
1.1.1.100 NADPH
-
 Carthamus tinctorius
1.1.1.100 NADPH
-
 Cuphea lutea