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Literature summary extracted from

  • Sheldon, P.S.; Kekwick, R.G.O.; Smith, C.G.; Sidebottom, C.; Slabas, A.R.
    3-Oxoacyl-[ACP] reductase from oil seed rape (Brassica napus) (1992), Biochim. Biophys. Acta, 1130, 151-159.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.100 inactivation by dilution can be partly prevented by inclusion of NADPH Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.100 0.003
-
 acetoacetyl-[acyl-carrier protein]
-
 Brassica napus
1.1.1.100 0.023
-
 NADPH + acetoacetyl-[acyl-carrier-protein] Brassica napus
1.1.1.100 0.035
-
 N-(2-sulfanylethyl)acetamide
-
 Brassica napus
1.1.1.100 0.261
-
 acetoacetyl-CoA
-
 Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 120000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Brassica napus
-
 oilseed
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100
-
 Brassica napus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.100 136000
-
 purified enzyme, protein determination by calculation from absorption at 280 nm Brassica napus

Storage Stability

EC Number Storage Stability Organism
1.1.1.100 27°C, 100 mM potassium phosphate buffer, 13% loss of activity after 24 h Brassica napus
1.1.1.100 4°C, 100 mM potassium phosphate, 52% loss of activity after 24 h Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 3-oxoacyl-[acyl-carrier protein] + NADPH
-
 Brassica napus (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Brassica napus D-beta-hydroxybutyryl-CoA + NADP+
-
 ?
1.1.1.100 acetoacetyl-N-cysteamine + NADPH
-
 Brassica napus D-3-hydroxybutyryl-N-cysteamine + NADP+
-
 ?
1.1.1.100 acetoacetyl-[acyl-carrier protein] + NADPH
-
 Brassica napus D-beta-hydroxybutyryl-[acyl-carrier protein] + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 tetramer 4 * 20000-30000, SDS-PAGE Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Brassica napus