BRENDA - Enzyme Database

Isolation and characterization of three alcohol dehydrogenase isozymes from Syrian golden hamster

Keung, W.M.; Alcohol. Clin. Exp. Res. 20, 213-220 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
1,10-phenanthroline
-
Mesocricetus auratus
1.1.1.1
4-Methylpyrazole
isoenzyme AA-ADH, BB-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
Biochanin A
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
daidzein
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
genistein
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
testosterone
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.02
-
12-hydroxydodecanoate
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.02
-
all-trans-retinol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.02
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.03
-
all-trans-retinol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.03
-
n-butanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.04
-
Cyclohexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.04
-
n-Hexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.05
-
12-hydroxydodecanoate
isoenzyme BB-ADH and isoenzyme ADH-TT
Mesocricetus auratus
1.1.1.1
0.05
-
all-trans-retinol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.06
-
12-hydroxydodecanoate
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.07
-
n-butanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.26
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.5
-
ethanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.76
-
Cyclohexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.76
-
ethanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.3
-
12-hydroxydodecanoate
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
2.6
-
n-butanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
28
-
ethanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
320
-
Cyclohexanol
isoenzyme TT-ADH
Mesocricetus auratus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Zinc
isoenzyme AA-ADH contains 4.3 zinc atoms per dimeric molecule, isoenzyme BB-ADH contains 3.7 zinc atoms per dimeric molecule, isoenzyme AA-ADH contains 4.1 zinc atoms per dimeric molecule
Mesocricetus auratus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
40000
-
2 * 40000, SDS-PAGE
Mesocricetus auratus
1.1.1.1
80000
-
gel filtration
Mesocricetus auratus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Mesocricetus auratus
-
3 major isoenzymes: TT-ADH, AA-ADH and BB-ADH
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
isoenzyme AA-ADH and BB-ADH most abundant in
Mesocricetus auratus
-
1.1.1.1
testis
isoenzyme TT-ADH is only found in testis
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
2.1
-
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.5
-
isoenzyme BB-ADH
Mesocricetus auratus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285650
Mesocricetus auratus
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
3beta-hydroxy-5beta-androstan-17-one + NAD+
catalyzed by isoenzyme BB-ADH, no activity with isoenzyme AA-ADH and TT-ADH
285650
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH
-
-
-
?
1.1.1.1
all-trans-retinol + NAD+
-
285650
Mesocricetus auratus
all-trans-retinal + NADH
-
-
-
?
1.1.1.1
butan-2-ol + NAD+
-
285650
Mesocricetus auratus
butan-2-one + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285650
Mesocricetus auratus
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285650
Mesocricetus auratus
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285650
Mesocricetus auratus
acetaldehyde + NADH
-
-
-
?
1.1.1.1
hexanol + NAD+
-
285650
Mesocricetus auratus
n-hexanal + NADH
-
-
-
-
1.1.1.1
methylcrotonyl alcohol + NAD+
-
285650
Mesocricetus auratus
methylcrotonaldehyde + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 40000, SDS-PAGE
Mesocricetus auratus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.35
-
all-trans retinol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
1.03
-
12-hydroxydodecanoate
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
1.3
-
all-trans retinol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
1.75
-
Cyclohexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
1.9
-
all-trans retinol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.2
-
12-hydroxydodecanoate
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.35
-
n-Hexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.4
-
Cyclohexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.58
-
n-butanol
isoenzymee BB-ADH
Mesocricetus auratus
1.1.1.1
2.63
-
methylcrotonyl alcohol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.77
-
12-hydroxydodecanoate
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.8
-
n-Hexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.83
-
ethanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3
-
methylcrotonyl alcohol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3.33
-
n-butanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3.38
-
ethanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
4.08
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
7.18
-
n-butanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
8.5
-
Cyclohexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
8.58
-
methylcrotonyl alcohol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
9.58
-
ethanol
isoenzyme TT-ADH
Mesocricetus auratus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Mesocricetus auratus
1.1.1.1
NADH
-
Mesocricetus auratus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Mesocricetus auratus
1.1.1.1
NADH
-
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
1,10-phenanthroline
-
Mesocricetus auratus
1.1.1.1
4-Methylpyrazole
isoenzyme AA-ADH, BB-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
Biochanin A
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
daidzein
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
genistein
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
1.1.1.1
testosterone
inhibition of isoenzyme BB-ADH, no inhibition of isoenzyme AA-ADH and TT-ADH
Mesocricetus auratus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.02
-
12-hydroxydodecanoate
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.02
-
all-trans-retinol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.02
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.03
-
all-trans-retinol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.03
-
n-butanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.04
-
Cyclohexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.04
-
n-Hexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
0.05
-
12-hydroxydodecanoate
isoenzyme BB-ADH and isoenzyme ADH-TT
Mesocricetus auratus
1.1.1.1
0.05
-
all-trans-retinol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.06
-
12-hydroxydodecanoate
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.07
-
n-butanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.26
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
0.5
-
ethanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.76
-
Cyclohexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
0.76
-
ethanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.3
-
12-hydroxydodecanoate
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
2.6
-
n-butanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
28
-
ethanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
320
-
Cyclohexanol
isoenzyme TT-ADH
Mesocricetus auratus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Zinc
isoenzyme AA-ADH contains 4.3 zinc atoms per dimeric molecule, isoenzyme BB-ADH contains 3.7 zinc atoms per dimeric molecule, isoenzyme AA-ADH contains 4.1 zinc atoms per dimeric molecule
Mesocricetus auratus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
40000
-
2 * 40000, SDS-PAGE
Mesocricetus auratus
1.1.1.1
80000
-
gel filtration
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
isoenzyme AA-ADH and BB-ADH most abundant in
Mesocricetus auratus
-
1.1.1.1
testis
isoenzyme TT-ADH is only found in testis
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
2.1
-
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.5
-
isoenzyme BB-ADH
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285650
Mesocricetus auratus
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
3beta-hydroxy-5beta-androstan-17-one + NAD+
catalyzed by isoenzyme BB-ADH, no activity with isoenzyme AA-ADH and TT-ADH
285650
Mesocricetus auratus
5beta-androstan-3,17-dione + NADH
-
-
-
?
1.1.1.1
all-trans-retinol + NAD+
-
285650
Mesocricetus auratus
all-trans-retinal + NADH
-
-
-
?
1.1.1.1
butan-2-ol + NAD+
-
285650
Mesocricetus auratus
butan-2-one + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285650
Mesocricetus auratus
butyraldehyde + NADH
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
285650
Mesocricetus auratus
cyclohexanone + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
-
285650
Mesocricetus auratus
acetaldehyde + NADH
-
-
-
?
1.1.1.1
hexanol + NAD+
-
285650
Mesocricetus auratus
n-hexanal + NADH
-
-
-
-
1.1.1.1
methylcrotonyl alcohol + NAD+
-
285650
Mesocricetus auratus
methylcrotonaldehyde + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 40000, SDS-PAGE
Mesocricetus auratus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.35
-
all-trans retinol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
1.03
-
12-hydroxydodecanoate
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
1.3
-
all-trans retinol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
1.75
-
Cyclohexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
1.9
-
all-trans retinol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.2
-
12-hydroxydodecanoate
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.35
-
n-Hexanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.4
-
Cyclohexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.58
-
n-butanol
isoenzymee BB-ADH
Mesocricetus auratus
1.1.1.1
2.63
-
methylcrotonyl alcohol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
2.77
-
12-hydroxydodecanoate
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.8
-
n-Hexanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
2.83
-
ethanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3
-
methylcrotonyl alcohol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3.33
-
n-butanol
isoenzyme AA-ADH
Mesocricetus auratus
1.1.1.1
3.38
-
ethanol
isoenzyme BB-ADH
Mesocricetus auratus
1.1.1.1
4.08
-
n-Hexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
7.18
-
n-butanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
8.5
-
Cyclohexanol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
8.58
-
methylcrotonyl alcohol
isoenzyme TT-ADH
Mesocricetus auratus
1.1.1.1
9.58
-
ethanol
isoenzyme TT-ADH
Mesocricetus auratus