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Literature summary extracted from
- Overexpression, purification and properties of alcohol dehydrogenase IV from Saccharomyces cerevisiae (1988), Biochim. Biophys. Acta, 950, 54-60.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | - |
Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 0.059 | - |
NAD+ | - |
Saccharomyces cerevisiae |  |
| 1.1.1.1 | 0.122 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 1.1.1.1 | 2.83 | - |
acetaldehyde | - |
Saccharomyces cerevisiae | |
| 1.1.1.1 | 16.7 | - |
ethanol | - |
Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | Zn2+ | activates | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 40000 | - |
2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
| 1.1.1.1 | 60000 | 80000 | gel filtration | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | Saccharomyces cerevisiae | - |
alcohol dehydrogenase IV | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | allyl alcohol + NAD+ | no activity | Saccharomyces cerevisiae | prop-2-en-1-al + NADH | - |
? | |
| 1.1.1.1 | butanol + NAD+ | weak | Saccharomyces cerevisiae | butyraldehyde + NADH | - |
? | |
| 1.1.1.1 | ethanol + NAD+ | - |
Saccharomyces cerevisiae | acetaldehyde + NADH | - |
? | |
| 1.1.1.1 | propan-2-ol + NAD+ | no activity | Saccharomyces cerevisiae | acetone + NADH | - |
? | |
| 1.1.1.1 | propanol + NAD+ | - |
Saccharomyces cerevisiae | propionaldehyde + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | dimer | 2 * 40000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 8.3 | - |
alcohol dehydrogenase IV | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 8.2 | 9.5 | pH 8.2: about 10% of maximal activity, pH 9.5: about 40% of maximal activity | Saccharomyces cerevisiae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | NAD+ | - |
Saccharomyces cerevisiae | |
| 1.1.1.1 | NADH | - |
Saccharomyces cerevisiae | |
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