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Literature summary extracted from

  • Indrati, R.; Ohita, Y.
    Purification and properties of alcohol dehydrogenase from a mutant strain of Candida guilliermondii (1992), Can. J. Microbiol., 38, 953-957.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 acetaldehyde linear noncompetitive inhibition Meyerozyma guilliermondii
1.1.1.1 iodoacetic acid
-
 Meyerozyma guilliermondii
1.1.1.1 NADH linear competitive inhibition Meyerozyma guilliermondii
1.1.1.1 PCMB
-
 Meyerozyma guilliermondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.036
-
 NADH
-
 Meyerozyma guilliermondii
1.1.1.1 0.076
-
 NAD+
-
 Meyerozyma guilliermondii
1.1.1.1 0.194
-
 acetaldehyde
-
 Meyerozyma guilliermondii
1.1.1.1 4.68
-
 ethanol
-
 Meyerozyma guilliermondii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 38000
-
 4 * 38000, SDS-PAGE Meyerozyma guilliermondii
1.1.1.1 152000
-
 gel filtration Meyerozyma guilliermondii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Meyerozyma guilliermondii
-
-
-
1.1.1.1 Meyerozyma guilliermondii B10-05
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Meyerozyma guilliermondii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.1 a primary alcohol + NAD+ = an aldehyde + NADH + H+ oxidizes ethanol in an ordered bi-bi mechanism with NAD+ as the first substrate fixed Meyerozyma guilliermondii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 79.1
-
-
 Meyerozyma guilliermondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 acetaldehyde + NADH + H+
-
 Meyerozyma guilliermondii ethanol + NAD+
-
 r
1.1.1.1 acetaldehyde + NADH + H+
-
 Meyerozyma guilliermondii B10-05 ethanol + NAD+
-
 r
1.1.1.1 allyl alcohol + NAD+
-
 Meyerozyma guilliermondii prop-2-en-1-al + NADH
-
 ?
1.1.1.1 butan-2-ol + NAD+
-
 Meyerozyma guilliermondii butan-2-one + NADH
-
 ?
1.1.1.1 butanol + NAD+
-
 Meyerozyma guilliermondii butyraldehyde + NADH
-
 ?
1.1.1.1 cinnamyl alcohol + NAD+
-
 Meyerozyma guilliermondii cinnamaldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+
-
 Meyerozyma guilliermondii acetaldehyde + NADH + H+
-
 r
1.1.1.1 ethylene glycol + NAD+
-
 Meyerozyma guilliermondii ? + NADH
-
 ?
1.1.1.1 ethylene glycol + NAD+
-
 Meyerozyma guilliermondii B10-05 ? + NADH
-
 ?
1.1.1.1 hexan-1-ol + NAD+
-
 Meyerozyma guilliermondii n-hexanal + NADH
-
 ?
1.1.1.1 methanol + NAD+ no activity Meyerozyma guilliermondii formaldehyde + NADH + H+
-
 ?
1.1.1.1 methanol + NAD+ no activity Meyerozyma guilliermondii B10-05 formaldehyde + NADH + H+
-
 ?
1.1.1.1 octan-1-ol + NAD+
-
 Meyerozyma guilliermondii n-octanal + NADH
-
 ?
1.1.1.1 pentan-2-ol + NAD+
-
 Meyerozyma guilliermondii 2-pentanone + NADH
-
 ?
1.1.1.1 pentanol + NAD+
-
 Meyerozyma guilliermondii n-pentanal + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Meyerozyma guilliermondii acetone + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Meyerozyma guilliermondii B10-05 acetone + NADH
-
 ?
1.1.1.1 propanol + NAD+
-
 Meyerozyma guilliermondii propionaldehyde + NADH + H+
-
 ?
1.1.1.1 propanol + NAD+
-
 Meyerozyma guilliermondii B10-05 propionaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 38000, SDS-PAGE Meyerozyma guilliermondii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 50 55
-
 Meyerozyma guilliermondii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 -20
-
 loss of activity after 24 h Meyerozyma guilliermondii
1.1.1.1 40
-
 stable below Meyerozyma guilliermondii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 8 8.5
-
 Meyerozyma guilliermondii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Meyerozyma guilliermondii
1.1.1.1 NADH
-
 Meyerozyma guilliermondii