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Literature summary extracted from

  • Rudge, J.; Bickerstaff, G.F.
    Purification and properties of an alcohol dehydrogenase from Sporotrichum pulverulentum (1986), Enzyme Microb. Technol., 8, 120-124.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.0086
-
 acetaldehyde
-
 Sporotrichum pulverulentum
1.1.1.1 0.021
-
 NADH
-
 Sporotrichum pulverulentum
1.1.1.1 0.17
-
 NAD+
-
 Sporotrichum pulverulentum
1.1.1.1 2.5
-
 ethanol
-
 Sporotrichum pulverulentum
1.1.1.1 64
-
 ethanol
-
 Sporotrichum pulverulentum
1.1.1.1 64
-
 propan-2-ol
-
 Sporotrichum pulverulentum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 42000
-
 4 * 42000, SDS-PAGE Sporotrichum pulverulentum
1.1.1.1 176000
-
 non-denaturing PAGE Sporotrichum pulverulentum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Sporotrichum pulverulentum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Sporotrichum pulverulentum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 196.3
-
-
 Sporotrichum pulverulentum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 2-ethylhexan-1-ol + NAD+ weak activity Sporotrichum pulverulentum 2-ethylhexanal + NADH
-
 ?
1.1.1.1 2-methylpropan-1-ol + NAD+
-
 Sporotrichum pulverulentum 2-methyl-propan-1-one + NADH
-
 ?
1.1.1.1 acetaldehyde + NADH + H+
-
 Sporotrichum pulverulentum ethanol + NAD+
-
 r
1.1.1.1 butan-2-ol + NAD+
-
 Sporotrichum pulverulentum butan-2-one + NADH
-
 ?
1.1.1.1 ethanol + NAD+
-
 Sporotrichum pulverulentum acetaldehyde + NADH
-
 r
1.1.1.1 hexanol + NAD+
-
 Sporotrichum pulverulentum n-hexanal + NADH
-
 ?
1.1.1.1 methanol + NAD+ no activity Sporotrichum pulverulentum formaldehyde + NADH + H+
-
 ?
1.1.1.1 octan-1-ol + NAD+ weak activity Sporotrichum pulverulentum n-octanal + NADH
-
 ?
1.1.1.1 octan-2-ol + NAD+ weak activity Sporotrichum pulverulentum octan-2-one + NADH
-
 ?
1.1.1.1 pentan-2-ol + NAD+
-
 Sporotrichum pulverulentum 2-pentanone + NADH
-
 ?
1.1.1.1 pentanal + NAD+
-
 Sporotrichum pulverulentum pentanone + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Sporotrichum pulverulentum acetone + NADH
-
 ?
1.1.1.1 propanol + NAD+
-
 Sporotrichum pulverulentum propionaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 42000, SDS-PAGE Sporotrichum pulverulentum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.1 30
-
-
 Sporotrichum pulverulentum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.1 10 60 10°C: about 65% of maximal activity, 50°C: about 40% of maximal activity Sporotrichum pulverulentum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 5.6 6.5 oxidation of ethanol Sporotrichum pulverulentum
1.1.1.1 8 8.4 reduction of aldehyde Sporotrichum pulverulentum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.1 5.2 6.8 pH 5.2: about 35% of maximal activity, pH 6.8: about 55% of maximal activity, oxidation of ethanol Sporotrichum pulverulentum
1.1.1.1 7.2 9.2 pH 7.2: about 50% of maximal activity, pH 9.2: about 60% of maximal activity Sporotrichum pulverulentum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Sporotrichum pulverulentum
1.1.1.1 NADH
-
 Sporotrichum pulverulentum