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Literature summary extracted from
- Characterization of three isoenzymes of rat alcohol dehydrogenase. Tissue distribution and physical and enzymatic properties (1987), Eur. J. Biochem., 162, 179-189.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | 4-methoxypyrazole | 0.1-10 mM, ADH-2 is practically insensitive, ADH-3 is very sensitive | Rattus norvegicus |  |
| 1.1.1.1 | pyrazole | 0.1-10 mM, ADH-2 is practically insensitive, ADH-3 is very sensitive | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 0.0017 | - |
NADH | isoenzyme ADH-2, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.013 | - |
12-hydroxydodecanoate | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.025 | - |
1-Octanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.04 | - |
NAD+ | isoenzyme ADH-2, pH 10.0 and pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.05 | - |
benzyl alcohol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.05 | - |
NAD+ | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.08 | - |
1-Pentanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.1 | - |
NAD+ | isoenzyme ADH-3 | Rattus norvegicus | |
| 1.1.1.1 | 0.1 | - |
1-Octanol | isoenzyme ADH-2 | Rattus norvegicus | |
| 1.1.1.1 | 0.1 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.17 | - |
1-butanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.2 | - |
NAD+ | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.25 | - |
NAD+ | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.3 | - |
octanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.35 | - |
2-Buten-1-ol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.5 | - |
1-Octanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 0.51 | - |
1-Octanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.4 | - |
m-nitrobenzaldehyde | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 1.4 | - |
benzyl alcohol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.4 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.4 | - |
ethanol | isoenzyme ADH-3, pH 10.0 and pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 1.6 | - |
Octanol | isoenzyme ADH-2, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 2.2 | - |
Cyclohexanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 2.5 | - |
2-Buten-1-ol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 3 | - |
Butanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 3.1 | - |
1-Pentanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 3.5 | - |
octanal | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 17 | - |
1-butanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 60 | - |
2-Buten-1-ol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 78 | - |
1-Pentanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 220 | - |
Cyclohexanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 230 | - |
1-butanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
| 1.1.1.1 | 340 | - |
ethanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1900 | - |
Cyclohexanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 5000 | - |
ethanol | isoenzyme ADH-1, pH 7.5 | Rattus norvegicus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | Zinc | ADH-1 contains 3.9 mol of zinc per mol of subunit, ADH-2 contains 4.2 mol of zinc per mol of subunit | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 39000 | - |
2 * 39000, ADH-2, SDS-PAGE | Rattus norvegicus |
| 1.1.1.1 | 40000 | - |
2 * 40000, ADH-3, SDS-PAGE | Rattus norvegicus |
| 1.1.1.1 | 43000 | - |
2 * 43000, ADH-1, SDS-PAGE | Rattus norvegicus |
| 1.1.1.1 | 80000 | - |
gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.1 | ADH-1, ADH-2 and ADH-3 | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.1 | adrenal gland | - |
Rattus norvegicus | - |
| 1.1.1.1 | brain | - |
Rattus norvegicus | - |
| 1.1.1.1 | cornea | - |
Rattus norvegicus | - |
| 1.1.1.1 | epididymis | - |
Rattus norvegicus | - |
| 1.1.1.1 | heart | - |
Rattus norvegicus | - |
| 1.1.1.1 | intestine | - |
Rattus norvegicus | - |
| 1.1.1.1 | kidney | - |
Rattus norvegicus | - |
| 1.1.1.1 | liver | - |
Rattus norvegicus | - |
| 1.1.1.1 | lung | - |
Rattus norvegicus | - |
| 1.1.1.1 | muscle | - |
Rattus norvegicus | - |
| 1.1.1.1 | ovary | - |
Rattus norvegicus | - |
| 1.1.1.1 | pancreas | - |
Rattus norvegicus | - |
| 1.1.1.1 | spleen | - |
Rattus norvegicus | - |
| 1.1.1.1 | stomach | - |
Rattus norvegicus | - |
| 1.1.1.1 | testis | - |
Rattus norvegicus | - |
| 1.1.1.1 | thymus | - |
Rattus norvegicus | - |
| 1.1.1.1 | uterus | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 0.1 | - |
isoenzyme 4 | Rattus norvegicus |
| 1.1.1.1 | 0.12 | - |
isoenzyme 1 | Rattus norvegicus |
| 1.1.1.1 | 0.2 | - |
isoenzyme 3 | Rattus norvegicus |
| 1.1.1.1 | 0.62 | - |
isoenzyme 2 | Rattus norvegicus |
| 1.1.1.1 | 1.14 | - |
ADH-2 | Rattus norvegicus |
| 1.1.1.1 | 1.3 | - |
ADH-3 | Rattus norvegicus |
| 1.1.1.1 | 32.5 | - |
ADH-1 | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.1 | 12-hydroxydodecanoate + NAD+ | - |
Rattus norvegicus | 12-oxododecanoic acid + NADH | - |
? | |
| 1.1.1.1 | 2-butene-1-ol + NAD+ | - |
Rattus norvegicus | ? + NADH | - |
? | |
| 1.1.1.1 | 3-oxo-5beta-androstan-17beta-ol + NADH | - |
Rattus norvegicus | 3beta,17beta-dihydroxy-5beta-androstane + NAD+ | - |
? | |
| 1.1.1.1 | acetaldehyde + NADH + H+ | - |
Rattus norvegicus | ethanol + NAD+ | - |
? | |
| 1.1.1.1 | benzyl alcohol + NAD+ | oxidation with isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | benzaldehyde + NADH | - |
? | |
| 1.1.1.1 | butanol + NAD+ | pH 10.0: oxidized by ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | butyraldehyde + NADH | - |
? | |
| 1.1.1.1 | cyclohexanol + NAD+ | - |
Rattus norvegicus | cyclohexanone + NADH | - |
? | |
| 1.1.1.1 | ethanol + NAD+ | isoenzyme ADH-1 and ADH-3, no activity with isoenzyme ADH-2 | Rattus norvegicus | acetaldehyde + NADH | - |
? | |
| 1.1.1.1 | m-nitrobenzaldehyde + NADH + H+ | - |
Rattus norvegicus | m-nitrobenzyl alcohol + NAD+ | - |
? | |
| 1.1.1.1 | methanol + NAD+ | oxidized with ADH-3, no activity with ADH-1 and ADH-2 | Rattus norvegicus | formaldehyde + NADH + H+ | - |
? | |
| 1.1.1.1 | octan-1-ol + NAD+ | - |
Rattus norvegicus | n-octanal + NADH | - |
? | |
| 1.1.1.1 | octanal + NADH + H+ | - |
Rattus norvegicus | octanol + NAD+ | - |
? | |
| 1.1.1.1 | pentanol + NAD+ | - |
Rattus norvegicus | n-pentanal + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.1 | dimer | 2 * 43000, ADH-1, SDS-PAGE | Rattus norvegicus |
| 1.1.1.1 | dimer | 2 * 40000, ADH-3, SDS-PAGE | Rattus norvegicus |
| 1.1.1.1 | dimer | 2 * 39000, ADH-2, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.1 | 0.383 | - |
Cyclohexanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 0.833 | - |
1-butanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1 | - |
ethanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1 | - |
benzyl alcohol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1 | - |
1-Octanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.17 | - |
1-Pentanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.27 | - |
12-hydroxydodecanoate | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.5 | - |
Cyclohexanol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.67 | - |
2-Buten-1-ol | isoenzyme ADH-3, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 1.75 | - |
Cyclohexanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 2.33 | - |
1-Octanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 3.53 | - |
1-Pentanol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 3.83 | - |
12-hydroxydodecanoate | isoenzyme ADH-12, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 5.83 | - |
2-Buten-1-ol | isoenzyme ADH-2, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 20 | - |
12-hydroxydodecanoate | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 48.8 | - |
1-butanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 48.8 | - |
1-Pentanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 60.8 | - |
1-Octanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 62.7 | - |
ethanol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 89.7 | - |
benzyl alcohol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
| 1.1.1.1 | 213 | - |
2-Buten-1-ol | isoenzyme ADH-1, pH 10.0 | Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.1 | 10.7 | - |
oxidation of ethanol, isoenzyme 2, 3 and 4 | Rattus norvegicus |
| 1.1.1.1 | 11 | - |
oxidation of octanol | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.1 | NAD+ | - |
Rattus norvegicus | |
| 1.1.1.1 | NADH | - |
Rattus norvegicus | |
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