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Literature summary extracted from

  • Hoshino, T.; Ishigura, I.; Ohta, Y.
    Rabbit liver alcohol dehydrogenase: purification and properties (1985), J. Biochem., 97, 1163-1172.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.1 dialysis against 50 mM Tris-HCl buffer, stable after 5 h, 3% loss of activity after 1 day, 82% loss of activity after 6 days Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 1,10-phenanthroline
-
 Oryctolagus cuniculus
1.1.1.1 2,2'-dipyridyl
-
 Oryctolagus cuniculus
1.1.1.1 4-methoxypyrazole
-
 Oryctolagus cuniculus
1.1.1.1 Ag+
-
 Oryctolagus cuniculus
1.1.1.1 Cu2+
-
 Oryctolagus cuniculus
1.1.1.1 glutathione
-
 Oryctolagus cuniculus
1.1.1.1 Hg2+
-
 Oryctolagus cuniculus
1.1.1.1 iodoacetic acid
-
 Oryctolagus cuniculus
1.1.1.1 NEM
-
 Oryctolagus cuniculus
1.1.1.1 PCMB
-
 Oryctolagus cuniculus
1.1.1.1 pyrazole
-
 Oryctolagus cuniculus
1.1.1.1 Zn2+
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.00694
-
 NAD+ pH 7.5 Oryctolagus cuniculus
1.1.1.1 0.0531
-
 NAD+ pH 10.8 Oryctolagus cuniculus
1.1.1.1 0.45
-
 ethanol pH 10.8 Oryctolagus cuniculus
1.1.1.1 3.33
-
 ethanol pH 7.5 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc contains 3.8 mol of Zn per mol of protein Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 36000
-
 2 * 36000, SDS-PAGE Oryctolagus cuniculus
1.1.1.1 72000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 2.69
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.1.1.1 4°C, in presence of 1.0 mM dithiothreitol, stable for 10 days Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 butanol + NAD+
-
 Oryctolagus cuniculus butyraldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+
-
 Oryctolagus cuniculus acetaldehyde + NADH
-
 ?
1.1.1.1 methanol + NAD+ no activity at pH 7.5, slight activity at pH 10.8 Oryctolagus cuniculus formaldehyde + NADH + H+
-
 ?
1.1.1.1 pentanol + NAD+
-
 Oryctolagus cuniculus n-pentanal + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Oryctolagus cuniculus acetone + NADH
-
 ?
1.1.1.1 propanol + NAD+
-
 Oryctolagus cuniculus propionaldehyde + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 36000, SDS-PAGE Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 10.8
-
 and a second lower maximum at pH 7.5 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Oryctolagus cuniculus
1.1.1.1 NADH
-
 Oryctolagus cuniculus
1.1.1.1 NADP+ no activity as coenzyme Oryctolagus cuniculus