BRENDA - Enzyme Database

Physical and enzymatic properties of a class III isozyme of human liver alcohol dehydrogenase: chi-ADH

Wagner, F.W.; Pares, X.; Holmquist, B.; Vallee, B.L.; Biochemistry 23, 2193-2199 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
4-Methylpyrazole
class III enzyme is completely insensitive to inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.0025
-
NADH
-
Homo sapiens
1.1.1.1
0.0064
-
NADH
isoenzyme beta1,beta1
Homo sapiens
1.1.1.1
0.007
-
NADH
isoenzyme gamma1,gamma1
Homo sapiens
1.1.1.1
0.0074
-
NAD+
isoenzyme beta2,beta2
Homo sapiens
1.1.1.1
0.0079
-
NAD+
isoenzyme gamma1,gamma1
Homo sapiens
1.1.1.1
0.0087
-
NAD+
isoenzyme gamma2,gamma2
Homo sapiens
1.1.1.1
0.011
-
NADH
isoenzyme alpha,alpha
Homo sapiens
1.1.1.1
0.013
-
NAD+
isoenzyme alpha,alpha
Homo sapiens
1.1.1.1
0.025
-
NAD+
-
Homo sapiens
1.1.1.1
0.1
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
0.105
-
NADH
isoenzyme beta2,beta2
Homo sapiens
1.1.1.1
0.18
-
NAD+
isoenzyme beta1,beta1
Homo sapiens
1.1.1.1
1.2
-
Octanol
-
Homo sapiens
1.1.1.1
2.4
-
octanal
-
Homo sapiens
1.1.1.1
11
-
Vanillyl alcohol
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Zinc
contains 3.8 mol of Zn per mol of protein
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
40000
-
2 * 40000, SDS-PAGE
Homo sapiens
1.1.1.1
41000
-
2 * 41000, class III isoenzyme chi ADH, SDS-PAGE
Homo sapiens
1.1.1.1
82700
-
equilibrium sedimentation
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.1
Homo sapiens
-
class III isoenzyme; isoenzyme chi-ADH
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.1.1.1
class III isoenzyme chi-ADH
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
0.6
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.1
4°C, 10 mM HEPES buffer, 1 mM dithioerythritol, pH 7.5, stable for 2 weeks
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285571
Homo sapiens
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
17beta-hydroxyetiocholan-3-one + NAD+
-
285571
Homo sapiens
ethiocholan-3,17-dione + NADH
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
285571
Homo sapiens
benzaldehyde + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285571
Homo sapiens
butyraldehyde + NADH
-
-
-
?
1.1.1.1
digitose + NAD+
-
285571
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
class III isoenzyme chi-ADH oxidizes ethanol very poorly
285571
Homo sapiens
acetaldehyde + NADH
-
-
-
?
1.1.1.1
ethylene glycol + NAD+
class III isoenzyme chi-ADH shows no activity
285571
Homo sapiens
? + NADH
-
-
-
-
1.1.1.1
furfuryl alcohol + NAD+
-
285571
Homo sapiens
furfural + NADH
-
-
-
?
1.1.1.1
methanol + NAD+
class III isoenzyme chi-ADH shows no activity
285571
Homo sapiens
formaldehyde + NADH + H+
-
-
-
-
1.1.1.1
octan-2-ol + NAD+
-
285571
Homo sapiens
octan-2-one + NADH
-
-
-
?
1.1.1.1
octanal + NADH + H+
-
285571
Homo sapiens
octanol + NAD+
-
-
-
?
1.1.1.1
pentanal + NAD+
-
285571
Homo sapiens
pentanone + NADH
-
-
-
?
1.1.1.1
phenylalaninol + NAD+
-
285571
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
vanillyl alcohol + NAD+
-
285571
Homo sapiens
vanillin + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 40000, SDS-PAGE; 2 * 41000, class III isoenzyme chi ADH, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.245
-
Pentanol
-
Homo sapiens
1.1.1.1
0.467
-
Vanillyl alcohol
-
Homo sapiens
1.1.1.1
3.03
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
3.33
-
octanal
-
Homo sapiens
1.1.1.1
7.33
-
Octanol
-
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.1
NADH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.1
NAD+
-
Homo sapiens
1.1.1.1
NADH
-
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.1
4-Methylpyrazole
class III enzyme is completely insensitive to inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.0025
-
NADH
-
Homo sapiens
1.1.1.1
0.0064
-
NADH
isoenzyme beta1,beta1
Homo sapiens
1.1.1.1
0.007
-
NADH
isoenzyme gamma1,gamma1
Homo sapiens
1.1.1.1
0.0074
-
NAD+
isoenzyme beta2,beta2
Homo sapiens
1.1.1.1
0.0079
-
NAD+
isoenzyme gamma1,gamma1
Homo sapiens
1.1.1.1
0.0087
-
NAD+
isoenzyme gamma2,gamma2
Homo sapiens
1.1.1.1
0.011
-
NADH
isoenzyme alpha,alpha
Homo sapiens
1.1.1.1
0.013
-
NAD+
isoenzyme alpha,alpha
Homo sapiens
1.1.1.1
0.025
-
NAD+
-
Homo sapiens
1.1.1.1
0.1
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
0.105
-
NADH
isoenzyme beta2,beta2
Homo sapiens
1.1.1.1
0.18
-
NAD+
isoenzyme beta1,beta1
Homo sapiens
1.1.1.1
1.2
-
Octanol
-
Homo sapiens
1.1.1.1
2.4
-
octanal
-
Homo sapiens
1.1.1.1
11
-
Vanillyl alcohol
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.1
Zinc
contains 3.8 mol of Zn per mol of protein
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
40000
-
2 * 40000, SDS-PAGE
Homo sapiens
1.1.1.1
41000
-
2 * 41000, class III isoenzyme chi ADH, SDS-PAGE
Homo sapiens
1.1.1.1
82700
-
equilibrium sedimentation
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
class III isoenzyme chi-ADH
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.1
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.1
0.6
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.1
4°C, 10 mM HEPES buffer, 1 mM dithioerythritol, pH 7.5, stable for 2 weeks
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.1
12-hydroxydodecanoate + NAD+
-
285571
Homo sapiens
12-oxododecanoic acid + NADH
-
-
-
?
1.1.1.1
17beta-hydroxyetiocholan-3-one + NAD+
-
285571
Homo sapiens
ethiocholan-3,17-dione + NADH
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
-
285571
Homo sapiens
benzaldehyde + NADH
-
-
-
?
1.1.1.1
butanol + NAD+
-
285571
Homo sapiens
butyraldehyde + NADH
-
-
-
?
1.1.1.1
digitose + NAD+
-
285571
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
ethanol + NAD+
class III isoenzyme chi-ADH oxidizes ethanol very poorly
285571
Homo sapiens
acetaldehyde + NADH
-
-
-
?
1.1.1.1
ethylene glycol + NAD+
class III isoenzyme chi-ADH shows no activity
285571
Homo sapiens
? + NADH
-
-
-
-
1.1.1.1
furfuryl alcohol + NAD+
-
285571
Homo sapiens
furfural + NADH
-
-
-
?
1.1.1.1
methanol + NAD+
class III isoenzyme chi-ADH shows no activity
285571
Homo sapiens
formaldehyde + NADH + H+
-
-
-
-
1.1.1.1
octan-2-ol + NAD+
-
285571
Homo sapiens
octan-2-one + NADH
-
-
-
?
1.1.1.1
octanal + NADH + H+
-
285571
Homo sapiens
octanol + NAD+
-
-
-
?
1.1.1.1
pentanal + NAD+
-
285571
Homo sapiens
pentanone + NADH
-
-
-
?
1.1.1.1
phenylalaninol + NAD+
-
285571
Homo sapiens
? + NADH
-
-
-
?
1.1.1.1
vanillyl alcohol + NAD+
-
285571
Homo sapiens
vanillin + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
dimer
2 * 40000, SDS-PAGE; 2 * 41000, class III isoenzyme chi ADH, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.245
-
Pentanol
-
Homo sapiens
1.1.1.1
0.467
-
Vanillyl alcohol
-
Homo sapiens
1.1.1.1
3.03
-
12-hydroxydodecanoate
-
Homo sapiens
1.1.1.1
3.33
-
octanal
-
Homo sapiens
1.1.1.1
7.33
-
Octanol
-
Homo sapiens