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Literature summary extracted from

  • Brouquisse, R.; Weigel, P.; Rhodes, D.; Yocum, C.F.; Hanson, A.D.
    Evidence for a ferredoxin-dependent choline monooxygenase from spinach chloroplast stroma (1989), Plant Physiol., 90, 322-329.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.7 catalase stimulation of activity by countering negative effects of H2O2 produced by ferredoxin autoxidation Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.7 betaine 300 mM betaine give 50% inhibition Spinacia oleracea
1.14.15.7 Zn2+ 10 mM inhibits activity Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.7 0.1
-
 choline reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.7 chloroplast in stromal fraction of chloroplasts Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.7 Ca2+ 10 mM Ca2+ can replace Mg2+ Spinacia oleracea
1.14.15.7 Mg2+ maximum activity with more than 10 mM Spinacia oleracea
1.14.15.7 additional information Mn2+, Cu2+ or Co2+ have no effect on activity Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.7 45000
-
 SDS-PAGE Spinacia oleracea
1.14.15.7 98000
-
 gel filtration with Ultrogel AcA 34 Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.7 Spinacia oleracea
-
 spinach, cv. Savoy Hybrid 612
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.7 0.00025
-
 activity varies with leaf age Spinacia oleracea
1.14.15.7 0.00058
-
 after salinization of plants with 200 mM NaCl for two weeks Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.7 choline + O2 + reduced ferredoxin
-
 Spinacia oleracea betaine aldehyde hydrate + oxidized ferredoxin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.7 7.7
-
 reduced ferredoxin provided by NADPH/ferredoxin-NADP+ reductase or reconstituted chloroplasts Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.7 Ferredoxin ferredoxin is reduced via NADPH and ferredoxin-NADP+ reductase Spinacia oleracea
1.14.15.7 O2 half-maximal rate with a O2 partial pressure of 2kPa, corresponding to an concentration of 0.025 mM Spinacia oleracea