Literature summary extracted from
Delorme, C.; Piffeteau, A.; Sobrio, F.; Marquet, A.
Mechanism-based inactivation of bovine cytochrome P-45011beta by 18-unsaturated progesterone derivatives (1997), Eur. J. Biochem., 248, 252-260.
Application
EC Number |
Application |
Comment |
Organism |
---|
1.14.15.4 |
medicine |
inhibitors for P-45011beta, against aldosterone overproduction which leads to oedematous diseases and hypertension |
Bos taurus |
General Stability
EC Number |
General Stability |
Organism |
---|
1.14.15.4 |
deoxycorticosterone, protection of enzyme from inactivation by 18-ethynylprogesterone |
Bos taurus |
1.14.15.4 |
phospholipid vesicles, 0.2% soybean lecithin, added to purified P-45011beta for storage and during incubation |
Bos taurus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.15.4 |
18-ethynylprogesterone |
mechanism-based inhibition; progesterone analog, time-dependent pseudo-first-order inactivation, concentration dependent |
Bos taurus |
|
1.14.15.4 |
18-vinylprogesterone |
mechanism-based inhibition; progesterone analog, with NADPH, time and concentration dependent, irreversible, pseudo-first-order process, covalent binding to and destruction of prosthetic heme group, 5fold more effective than its acetylenic analog 18-ethynylprogesterone |
Bos taurus |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.15.4 |
mitochondrion |
- |
Bos taurus |
5739 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.15.4 |
Bos taurus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.15.4 |
copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system |
Bos taurus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
1.14.15.4 |
adrenal cortex |
- |
Bos taurus |
- |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.14.15.4 |
-80°C, after octyl-Sepharose, deoxycorticosterone-bound, 50 mM potassium phosphate, pH 7.4, 0.5% sodium cholate, 0.2% Tween 20, 0.01 mM 11-deoxycorticosterone, 0.1 mM dithiothreitol, 0.1 mM EDTA |
Bos taurus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.15.4 |
11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 |
3 step 11beta-/18-hydroxylation to aldosterone |
Bos taurus |
aldosterone + oxidized adrenal ferredoxin + 2 H2O |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.14.15.4 |
30 |
- |
assay at |
Bos taurus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.14.15.4 |
7.4 |
- |
assay at |
Bos taurus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.15.4 |
NADPH |
requirement during inactivation |
Bos taurus |
|
1.14.15.4 |
NADPH |
adrenodoxin and NADPH-linked adrenodoxin reductase |
Bos taurus |
|