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Literature summary extracted from

  • Tsubaki, M.; Ichikawa, Y.; Fujimoto, Y.; Yu, N.T.; Hori, H.
    Active site of bovine adrenocortical cytochrome P-45011beta studied by resonance Raman and electron paramagnetic resonance spectroscopies: distinction from cytochrome P-450scc (1990), Biochemistry, 29, 8805-8812.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.4 46000
-
 x * 46000 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 ultracentrifugation, ammonium sulfate precipitation, omega-aminooctyl-Sepharose, adrenodoxin-Sepharose, no glycerol used Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O ferric cytochrome P-45011beta-deoxycorticosterone complex classified as a pentacoordinated species, H2O molecule binds ferric heme iron Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 11beta-/18-hydroxylation Bos taurus aldosterone + oxidized adrenal ferredoxin + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.4 ? x * 46000 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH
-
 Bos taurus