EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | Anti-11beta-hydroxylase IgG | polyclonal, raised in rabbits, inhibition of 11-beta/18-hydroxylation and aldosterone synthesis of corticosterone | Bos taurus | |
1.14.15.4 | Metyrapone | - |
Bos taurus | |
1.14.15.4 | Metyrapone | inhibition of the purified 11beta-hydroxylase, 18-hydroxylation and aldosterone synthesis of corticosterone are inhibited | Sus scrofa | |
1.14.15.4 | SKF 525A | little inhibition of the purified 11beta-hydroxylase, 18-hydroxylation and aldosterone synthesis of corticosterone are inhibited | Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.4 | mitochondrion | from zona fasciculata | Bos taurus | 5739 | - |
1.14.15.4 | mitochondrion | from zona glomerulosa | Bos taurus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 46000 | - |
x * 46000, two-dimensional gel | Sus scrofa |
1.14.15.4 | 47500 | - |
x * 47500 | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.4 | Bos taurus | - |
- |
- |
1.14.15.4 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.4 | copurification of 11beta-/18-hydroxylase, cholate extraction, ammonium sulfate precipitation, Tween extraction, octymine-Sepharose | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.4 | adrenal cortex | - |
Sus scrofa | - |
1.14.15.4 | adrenal cortex | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 0.00003 | - |
18-hydroxylation | Bos taurus |
1.14.15.4 | 0.0053 | - |
11beta-hydroxylation | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | - |
Bos taurus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | supported by adrenodoxin and adrenodoxin reductase, 3 step-hydroxylation with corticosterone and 18-hydroxycorticosterone as intermediates, 11beta-/18-hydroxylase | Sus scrofa | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
1.14.15.4 | corticosterone + reduced adrenal ferredoxin + O2 | 18-hydroxycorticosterone as intermediate of 11beta-hydroxylation | Bos taurus | aldosterone + oxidized adrenal ferredoxin + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.15.4 | ? | x * 46000, two-dimensional gel | Sus scrofa |
1.14.15.4 | ? | x * 47500 | Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 30 | - |
assay at | Sus scrofa |
1.14.15.4 | 30 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | NADPH | - |
Sus scrofa | |
1.14.15.4 | NADPH | - |
Bos taurus |