Literature summary extracted from

Lombardo, A.; Defaye, G.; Guidicelli, C.; Monnier, N.; Chambaz, E.M.
Integration of purified adrenocortical cytochrome P-45011beta into phospholipid vesicles (1982), Biochem. Biophys. Res. Commun., 104, 1638-1645.

General Stability

EC Number	General Stability	Organism
1.14.15.4	inclusion into phospholipid vesicles stabilizes	Bos taurus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.14.15.4	mitochondrion	cytochrome P-45011beta in inner mitochondrial membrane, adrenodoxin reductase and adrenodoxin are loosely associated, inclusion of purified P-45011beta into phospholipid vesicles mimics the situation in vivo	Bos taurus	5739	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.15.4	48000	-	x * 48000, SDS-PAGE	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.15.4	Bos taurus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.15.4	ammonium sulfate precipitation, octyl-Sepharose adsorption with cholate and phosphotidylcholine extraction	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.15.4	adrenal cortex	-	Bos taurus	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.14.15.4	1.35	1.36	corticosterone, enzyme incorporated into liposomes	Bos taurus
1.14.15.4	1.38	-	corticosterone, soluble form of enzyme	Bos taurus

Storage Stability

EC Number	Storage Stability	Organism
1.14.15.4	4°C, enzyme incorporated into liposomes, stable during 2 weeks	Bos taurus
1.14.15.4	4°C, soluble form, loses part of activity in few days, after 2 weeks 1/4 of original activity observed	Bos taurus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.15.4	11-deoxycorticosterone + reduced adrenal ferredoxin + O2	11beta-hydroxylation	Bos taurus	corticosterone + oxidized adrenal ferredoxin + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.15.4	?	x * 48000, SDS-PAGE	Bos taurus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.14.15.4	37	-	assay at	Bos taurus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.14.15.4	7.4	-	assay at	Bos taurus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.15.4	NADPH	-	Bos taurus

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Release 2023.1 (January 2023)