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Literature summary extracted from
- Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex (1989), J. Biol. Chem., 264, 10935-10938.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.4 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 49500 | - |
x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
| 1.14.15.4 | 51500 | - |
x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | Rattus norvegicus | - |
male, Sprague-Dawley, low sodium, high potassium treated | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.4 | cholate extraction, hydroxyapatite HPLC | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.4 | adrenal cortex | capsular portion in zona glomerulosa cells, decapsular in zonae fasciculata and reticularis | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | additional information | - |
several activities of 49.5 and 51.5 kDa proteins for multihydroxylation steps | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | 49 kDa protein, exclusively in zona glomerulosa, 11beta -hydroxylation, as well as 18-hydroxylation and 18-hydroxydehydrogenation of corticosterone with 18-hydroxydeoxycorticosterone and 18-hydroxycorticosterone as intermediates | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? |  |
| 1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + 2 H+ + 2 O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | aldosterone + oxidized adrenal ferredoxin + 2 H2O | - |
? | |
| 1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? | |
| 1.14.15.4 | 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | by 49.5 kDa protein from capsular portion, 11beta-/18-hydroxylation by 51.5 kDa protein | Rattus norvegicus | corticosterone + oxidized adrenal ferredoxin + H2O | 51.5 kDa protein | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.15.4 | ? | x * 49500 of capsular portion and x * 51500 of capsular and decapsular portion, 11beta-hydroxylase activities, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.4 | 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.4 | NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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