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Literature summary extracted from

  • Watanuki, M.; Tilley, B.E.; Hall, P.F.
    Purification and properties of cytochrome p-450 (11beta- and 18-hydroxylase) from bovine adrenocortical mitochondria (1977), Biochim. Biophys. Acta, 483, 236-247.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.15.4 deoxycorticosterone stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.4 CO
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.4 0.02
-
 deoxycorticosterone identical for both enzymes activities Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.4 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.4 47500
-
 4 * 47500, SDS-PAGE Bos taurus
1.14.15.4 47500
-
 x * 47500, SDS-PAGE Bos taurus
1.14.15.4 185000
-
 exclusion chromatography Bos taurus
1.14.15.4 1000000
-
 glycerol density gradient Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.4 additional information Bos taurus corticosterone generally believed to be preferred substrate for 18-hydroxylase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.4 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.4 copurification of 11beta-hydroxylase with 18-hydroxylase, ammonium sulfate, DEAE-chromatography Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.4 a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O both hydroxylation activities seem to occur in a single enzyme Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.4 adrenal cortex
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.4 0.001061
-
 deoxycorticosterone, 18-hydroxylation activity in Bos taurus
1.14.15.4 0.01193
-
 deoxycorticosterone, 11beta-hydroxylation activity Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.14.15.4 -20°C, 50% glycerol, 1 week, without significant loss of activity Bos taurus
1.14.15.4 4°C, 1 week, without significant loss of activity Bos taurus
1.14.15.4 4°C, sonicated mitochondria, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.4 11-deoxycorticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus corticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 corticosterone + reduced adrenal ferredoxin + O2 addition of adrenodoxin and adrenodoxin reductase Bos taurus 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
 ?
1.14.15.4 additional information corticosterone generally believed to be preferred substrate for 18-hydroxylase Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.4 ? x * 47500, SDS-PAGE Bos taurus
1.14.15.4 tetramer 4 * 47500, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.4 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.4 7
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.4 NADPH
-
 Bos taurus