EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.154 | lanosterol | induction and activation | Saccharomyces cerevisiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.154 | anti-P-45014DM antibodies | complete inhibition | Saccharomyces cerevisiae | |
1.14.14.154 | CO | ratio CO:O2 of 90:10, 51.1% inhibition, ratio CO:O2 of 95:5, 100% inhibition | Saccharomyces cerevisiae | |
1.14.14.154 | menadione | 0.125 mM: 62.1% inhibition | Saccharomyces cerevisiae | |
1.14.14.154 | Metyrapone | strong inhibition, 0.1 mM: 57.3% inhibition | Saccharomyces cerevisiae | |
1.14.14.154 | nitrogen | nitrogen atmosphere | Saccharomyces cerevisiae | |
1.14.14.154 | SKF-525A | potent inhibitor, 1.0 mM: 100% inhibition | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.154 | additional information | - |
additional information | kinetics | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.154 | microsome | - |
Saccharomyces cerevisiae | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | natural substrate | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | lanosta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | P-45014DM catalyzes all three oxygenation steps from lanosterol to dimethylcholestratrienol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Saccharomyces cerevisiae | ergosterol synthesis in yeast involves oxidative removal of the 14alpha-methyl group, C-32, of lanosterol | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.154 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.154 | - |
Saccharomyces cerevisiae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.154 | a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | mechanism | Saccharomyces cerevisiae | |
1.14.14.154 | a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | stoichiometry | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | natural substrate | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | lanosta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3beta-ol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | P-45014DM catalyzes all three oxygenation steps from lanosterol to dimethylcholestratrienol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | ergosterol synthesis in yeast involves oxidative removal of the 14alpha-methyl group, C-32, of lanosterol | Saccharomyces cerevisiae | 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.154 | additional information | reaction reqires molecular oxygen, does not occur anaerobically | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.154 | 30 | - |
assay at | Saccharomyces cerevisiae |
1.14.14.154 | 30 | - |
aerobic conditions | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.154 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.154 | cytochrome P450 | - |
Saccharomyces cerevisiae | |
1.14.14.154 | heme | heme-thiolate enzyme | Saccharomyces cerevisiae |