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Literature summary extracted from

  • Haddock, J.D.; Gibson, D.T.
    Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400 (1995), J. Bacteriol., 177, 5834-5839.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.18 Fe2+ omission of ferrous ammonium sulfate reduces specific activity of purified ISP 7.4fold Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.18 27300
-
 beta subunit of ISP, SDS-PAGE Pseudomonas sp.
1.14.12.18 53000
-
 alpha subunit of ISP, SDS-PAGE Pseudomonas sp.
1.14.12.18 209000
-
 native protein, gel filtration with Superose 12 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Pseudomonas sp.
-
-
-
1.14.12.18 Pseudomonas sp. LB400
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.18 purification of the iron sulfur protein (ISP) of biphenyl 2,3-dioxygenase Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Pseudomonas sp. cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 biphenyl + NAD(P)H + O2
-
 Pseudomonas sp. LB400 cis-biphenyl 2,3-dihydrodiol + NAD(P)+
-
 ?
1.14.12.18 carbazole + NADH + O2 no products with carbazole detected Pseudomonas sp. 3-hydroxycarbazole + NAD+
-
 ?
1.14.12.18 carbazole + NADH + O2 no products with carbazole detected Pseudomonas sp. LB400 3-hydroxycarbazole + NAD+
-
 ?
1.14.12.18 additional information no substrates are benzene, toluene, 2,5-dichlorotoluene, carbazole and dibenzothiophene Pseudomonas sp. ?
-
 ?
1.14.12.18 additional information no substrates are benzene, toluene, 2,5-dichlorotoluene, carbazole and dibenzothiophene Pseudomonas sp. LB400 ?
-
 ?
1.14.12.18 naphthalene + NADPH + O2 poor substrate Pseudomonas sp. cis-naphthalene 1,2-dihydrodiol + NADP+
-
 ?
1.14.12.18 naphthalene + NADPH + O2 poor substrate Pseudomonas sp. LB400 cis-naphthalene 1,2-dihydrodiol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.18 heterohexamer alpha3,beta3, 3 * 53000 + 3 * 27300, measurement of molecular weight and Stokes' radius with gel filtration Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 Ferredoxin
-
 Pseudomonas sp.