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Literature summary extracted from
- Preparation and characterization of immobilized glucose-phosphate isomerase (1986), Enzyme Microb. Technol., 8, 222-226.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.3.1.9 | immobilization increases the stability against urea | Oryctolagus cuniculus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.9 | 0.1 | - |
fructose 6-phosphate | glucose 6-phosphate, soluble enzyme | Oryctolagus cuniculus |  |
| 5.3.1.9 | 0.2 | - |
fructose 6-phosphate | glucose 6-phosphate, immobilized enzyme | Oryctolagus cuniculus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.9 | Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.1.9 | muscle | - |
Oryctolagus cuniculus | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.1.9 | 8-10°C, storage stability of enzyme immobilized on different carriers, overview | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.9 | Glucose 6-phosphate | - |
Oryctolagus cuniculus | Fructose 6-phosphate | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 50 | - |
immobilized enzyme | Oryctolagus cuniculus |
| 5.3.1.9 | 50 | 55 | soluble enzyme | Oryctolagus cuniculus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 30 | 60 | 30°C: about 45% of maximal activity of immobilized enzyme, about 35% of maximal activity of soluble enzyme, 60°C: about 60% of maximal activity of immobilized enzyme, about 65% of maximal activity of immobilized enzyme | Oryctolagus cuniculus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 45 | - |
soluble enzyme, t1/2: 42 min. Immobilized enzyme, t1/2: 82 min | Oryctolagus cuniculus |
| 5.3.1.9 | 50 | - |
soluble enzyme, t1/2: 12 min. Immobilized enzyme, t1/2: 10 min | Oryctolagus cuniculus |
| 5.3.1.9 | 55 | - |
soluble enzyme, t1/2: 2 min. Immobilized enzyme, t1/2: 1 min | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 8 | - |
soluble and immobilized enzyme | Oryctolagus cuniculus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 6 | 10 | pH 6.0: about 70% of maximal activity of soluble enzyme, about 30% of maximal activity of immobilized enzyme | Oryctolagus cuniculus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.9 | 6 | - |
immobilized enzyme: no marked change in the first few hours at different pH levels from pH 6.0-9.0, after a longer incubation the highest stability is measured at pH 6.0 | Oryctolagus cuniculus |
| 5.3.1.9 | 8 | - |
highest stability of soluble enzyme | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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