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Literature summary extracted from

  • Smith, C.A.; Rangarajan, M.; Hartley, B.S.
    D-Xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728 (1991), Biochem. J., 277, 255-261.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 Ca2+ strict competitive against Mg2+ Arthrobacter sp.
5.3.1.5 D-sorbitol mixed-type inhibition Arthrobacter sp.
5.3.1.5 D-xylitol competitive Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 3.3
-
 D-xylose D-xylose, 30°C Arthrobacter sp.
5.3.1.5 86
-
 D-glucose D-lyxose, 30°C Arthrobacter sp.
5.3.1.5 170
-
 D-fructose 30°C Arthrobacter sp.
5.3.1.5 190
-
 D-ribose 30°C Arthrobacter sp.
5.3.1.5 210
-
 D-glucose 60°C Arthrobacter sp.
5.3.1.5 225
-
 D-glucose 30°C Arthrobacter sp.
5.3.1.5 250
-
 D-glucose D-fructose, 60°C Arthrobacter sp.
5.3.1.5 280
-
 L-arabinose 30°C Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ less effective than Mg2+ in D-xylose isomerization Arthrobacter sp.
5.3.1.5 Mg2+ most effective in activation of D-xylose isomerization Arthrobacter sp.
5.3.1.5 Mn2+ less effective than Mg2+ in activation of D-xylose isomerization Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 47000
-
 4 * 47000, SDS-PAGE Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Arthrobacter sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 12.5
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Arthrobacter sp. D-Fructose
-
 ?
5.3.1.5 D-Lyxose
-
 Arthrobacter sp. ?
-
 ?
5.3.1.5 D-Ribose
-
 Arthrobacter sp. ?
-
 ?
5.3.1.5 D-Xylose
-
 Arthrobacter sp. D-Xylulose
-
 ?
5.3.1.5 L-Arabinose poor substrate Arthrobacter sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 tetramer 4 * 47000, SDS-PAGE Arthrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 0.0617
-
 D-Lyxose 30°C Arthrobacter sp.
5.3.1.5 0.515
-
 D-ribose 30°C Arthrobacter sp.
5.3.1.5 1.31
-
 L-arabinose 30°C Arthrobacter sp.
5.3.1.5 1.67
-
 D-fructose 30°C Arthrobacter sp.
5.3.1.5 2.32
-
 D-glucose 30°C Arthrobacter sp.
5.3.1.5 8.88
-
 D-xylose 30°C Arthrobacter sp.
5.3.1.5 19.9
-
 D-glucose 60°C Arthrobacter sp.
5.3.1.5 20.5
-
 D-fructose 60°C Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 8 8.5 D-fructose Arthrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 5.5 9.5 pH 5.5: about 40% of maximal activity, pH 9.5: about 80% of maximal activity, D-fructose isomerization Arthrobacter sp.