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Literature summary extracted from

  • Kwon, H.J.; Kitada, M.; Horikoshi, K.
    Purification and properties of the D-xylose isomerase from alkalophilic Bacillus No. KX-6 (1987), Agric. Biol. Chem., 51, 1983-1989.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 Ag+
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 Cu2+
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 Fe2+
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 Hg2+
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 Pb2+
-
 Bacillus sp. (in: Bacteria)
5.3.1.5 Zn2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 0.076
-
 D-xylose
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ stimulates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 58000
-
 2 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)
5.3.1.5 120000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Bacillus sp. (in: Bacteria)
-
 No. KX-6
-
5.3.1.5 Bacillus sp. (in: Bacteria) No. KX-6
-
 No. KX-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 7.5
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Xylose
-
 Bacillus sp. (in: Bacteria) D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Bacillus sp. (in: Bacteria) No. KX-6 D-Xylulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 dimer 2 * 58000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.3.1.5 40 80 40°C: about 40% of maximal activity, 80°C, about 30% of maximal activity Bacillus sp. (in: Bacteria)
5.3.1.5 50 70 50°C: about 55% of maximal activity, 70°C: about 65% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 50
-
 pH 7.5, 30 min, stable up to Bacillus sp. (in: Bacteria)
5.3.1.5 60
-
 pH 7.5, 30 min, about 35% loss of activity Bacillus sp. (in: Bacteria)
5.3.1.5 70
-
 about 65% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7 10
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.5 6 11 pH 6: about 50% of maximal activity, pH 11: about 65% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.5 6 11 40°C, 30 min, stable Bacillus sp. (in: Bacteria)