BRENDA - Enzyme Database

Properties of D-xylose isomerase from Streptomyces albus

Sanchez, S.; Smiley, K.L.; Appl. Microbiol. 29, 745-750 (1975)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
5.3.1.5
Mg2+ and Co2+ protect from thermal denaturation
Streptomyces albus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.5
D-sorbitol
competitive against D-glucose, D-xylose, and D-ribose. Co2+ and Mg2+ prevent inhibition
Streptomyces albus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.5
86
-
D-glucose
-
Streptomyces albus
5.3.1.5
153
-
L-arabinose
-
Streptomyces albus
5.3.1.5
312
-
L-rhamnose
-
Streptomyces albus
5.3.1.5
350
-
D-ribose
-
Streptomyces albus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.1.5
Co2+
1.0 mM, partial activation, Km: 0.003 mM; the binding sites for Co2+ and Mn2+ are different from each other
Streptomyces albus
5.3.1.5
Mg2+
1.0 mM, strong activation, Km: 0.03 mM; the binding sites for Co2+ and Mn2+ are different from each other
Streptomyces albus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.1.5
Streptomyces albus
-
NRRL 5778
-
5.3.1.5
Streptomyces albus NRRL 5778
-
NRRL 5778
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.5
D-Glucose
-
2735
Streptomyces albus
D-Fructose
-
-
-
-
5.3.1.5
D-Glucose
-
2735
Streptomyces albus NRRL 5778
D-Fructose
-
-
-
-
5.3.1.5
D-Ribose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
D-Ribose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
5.3.1.5
D-Xylose
-
2735
Streptomyces albus
D-Xylulose
-
2735
Streptomyces albus
-
5.3.1.5
D-Xylose
-
2735
Streptomyces albus NRRL 5778
D-Xylulose
-
2735
Streptomyces albus NRRL 5778
-
5.3.1.5
L-Arabinose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
L-Arabinose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
5.3.1.5
L-Rhamnose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
L-Rhamnose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.1.5
70
80
-
Streptomyces albus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.5
7
9
-
Streptomyces albus
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.1.5
Mg2+ and Co2+ protect from thermal denaturation
Streptomyces albus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.1.5
D-sorbitol
competitive against D-glucose, D-xylose, and D-ribose. Co2+ and Mg2+ prevent inhibition
Streptomyces albus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.1.5
86
-
D-glucose
-
Streptomyces albus
5.3.1.5
153
-
L-arabinose
-
Streptomyces albus
5.3.1.5
312
-
L-rhamnose
-
Streptomyces albus
5.3.1.5
350
-
D-ribose
-
Streptomyces albus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.3.1.5
Co2+
1.0 mM, partial activation, Km: 0.003 mM; the binding sites for Co2+ and Mn2+ are different from each other
Streptomyces albus
5.3.1.5
Mg2+
1.0 mM, strong activation, Km: 0.03 mM; the binding sites for Co2+ and Mn2+ are different from each other
Streptomyces albus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.1.5
D-Glucose
-
2735
Streptomyces albus
D-Fructose
-
-
-
-
5.3.1.5
D-Glucose
-
2735
Streptomyces albus NRRL 5778
D-Fructose
-
-
-
-
5.3.1.5
D-Ribose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
D-Ribose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
5.3.1.5
D-Xylose
-
2735
Streptomyces albus
D-Xylulose
-
2735
Streptomyces albus
-
5.3.1.5
D-Xylose
-
2735
Streptomyces albus NRRL 5778
D-Xylulose
-
2735
Streptomyces albus NRRL 5778
-
5.3.1.5
L-Arabinose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
L-Arabinose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
5.3.1.5
L-Rhamnose
-
2735
Streptomyces albus
?
-
-
-
-
5.3.1.5
L-Rhamnose
-
2735
Streptomyces albus NRRL 5778
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.3.1.5
70
80
-
Streptomyces albus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.1.5
7
9
-
Streptomyces albus