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Literature summary extracted from

  • Izumori, K.; Rees, A.W.; Elbein, A.D.
    Purification, crystallization, and properties of D-ribose isomerase from Mycobacterium smegmatis (1975), J. Biol. Chem., 250, 8085-8087.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.20
-
 Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.20 L-arabitol
-
 Mycolicibacterium smegmatis
5.3.1.20 ribitol
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.20 4
-
 D-ribose
-
 Mycolicibacterium smegmatis
5.3.1.20 5.3
-
 L-Lyxose
-
 Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.20 Co2+ divalent cation required, Km: 0.0004 mM Mycolicibacterium smegmatis
5.3.1.20 Mg2+ divalent cation required, Km: 0.018 mM Mycolicibacterium smegmatis
5.3.1.20 Mn2+ divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.20 145000 174000 sedimentation equilibrium analysis Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.20 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.20
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.20 1.84
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.20 D-Allose
-
 Mycolicibacterium smegmatis ?
-
 ?
5.3.1.20 D-Lyxose
-
 Mycolicibacterium smegmatis ?
-
 ?
5.3.1.20 D-ribose
-
 Mycolicibacterium smegmatis D-ribulose
-
 ?
5.3.1.20 L-lyxose
-
 Mycolicibacterium smegmatis ?
-
 ?
5.3.1.20 L-Rhamnose
-
 Mycolicibacterium smegmatis L-Rhamnulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.20 tetramer 4 * 42000-44000, SDS-PAGE in presence of Mn2+ Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.20 7.5 8.5
-
 Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.20 6.5 9.5 pH 6.5: about 45% of maximal activity, pH 9.5: about 55% of maximal activity Mycolicibacterium smegmatis