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Literature summary extracted from

  • Bhuiyan, S.H.; Itami, Y.; Izumori, K.
    Isolation of an L-rhamnose isomerase-constitutive mutant of Pseudomonas sp. strain LL172: purification and characterization of the enzyme (1997), J. Ferment. Bioeng., 84, 319-323.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 55
-
 L-rhamnose
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Co2+ 30% of the activation compared to Mn2+ Pseudomonas sp.
5.3.1.14 Mg2+ 6% of the activiation compared to Mn2+ Pseudomonas sp.
5.3.1.14 Mn2+ activates Pseudomonas sp.
5.3.1.14 Mn2+ 1 mM MnCl2 enhances activity 4fold Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 42000
-
 4 * 42000, SDS-PAGE Pseudomonas sp.
5.3.1.14 150000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Pseudomonas sp.
-
-
-
5.3.1.14 Pseudomonas sp.
-
 L-lyxose-utilizing mutant that produces L-rhamnose isomerase constitutively
-
5.3.1.14 Pseudomonas sp. LL172
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 109
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose 10% of the activity relative to L-rhamnose Pseudomonas sp. D-psicose
-
 ?
5.3.1.14 D-Gulose 10% of the activity relative to L-rhamnose Pseudomonas sp. ?
-
 ?
5.3.1.14 D-ribose equilibrium ratio of D-ribose:D-ribulose is 70:30 Pseudomonas sp. D-ribulose
-
 ?
5.3.1.14 D-ribose 16% of the activity relative to L-rhamnose Pseudomonas sp. D-ribulose
-
 ?
5.3.1.14 L-lyxose equilibrium ratio of L-lyxose:L-xylulose is 70:30 Pseudomonas sp. L-xylulose
-
 ?
5.3.1.14 L-lyxose 97% of the activity relative to L-rhamnose Pseudomonas sp. L-xylulose
-
 ?
5.3.1.14 L-lyxose equilibrium ratio of L-lyxose:L-xylulose is 70:30 Pseudomonas sp. LL172 L-xylulose
-
 ?
5.3.1.14 L-lyxose 97% of the activity relative to L-rhamnose Pseudomonas sp. LL172 L-xylulose
-
 ?
5.3.1.14 L-Mannose 30% of the activity relative to L-rhamnose Pseudomonas sp. L-Fructose
-
 ?
5.3.1.14 L-Mannose equilibrium ratio of L-mannose:L-fructose is 30:70 Pseudomonas sp. L-Fructose
-
 ?
5.3.1.14 L-Mannose 30% of the activity relative to L-rhamnose Pseudomonas sp. LL172 L-Fructose
-
 ?
5.3.1.14 L-Mannose equilibrium ratio of L-mannose:L-fructose is 30:70 Pseudomonas sp. LL172 L-Fructose
-
 ?
5.3.1.14 L-Rhamnose
-
 Pseudomonas sp. L-Rhamnulose
-
 ?
5.3.1.14 L-Rhamnose
-
 Pseudomonas sp. LL172 L-Rhamnulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.14 tetramer 4 * 42000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 60
-
 pH 9.0, 10 min Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 60
-
 pH 9.0, 10 min, stable Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 9
-
 50°C Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.14 5 11 4°C, 24 h, stable Pseudomonas sp.
5.3.1.14 7 11 4°C, 24 h, pH 7: about 30% loss of activity, pH 9: stable, pH 11: about 10% loss of activity, free enzyme Pseudomonas sp.