EC Number | Application | Comment | Organism |
---|---|---|---|
5.3.1.14 | synthesis | large scale production of L-mannose from L-fructose by immobilized enzyme | Pseudomonas sp. |
EC Number | General Stability | Organism |
---|---|---|
5.3.1.14 | immobilized enzyme retains about 90% of the initial activity even after five repeated uses in a batch reaction | Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.14 | Mn2+ | immobilized as well as free enzyme requires Mn2+ for maximal activity | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.14 | Pseudomonas sp. | - |
- |
- |
5.3.1.14 | Pseudomonas sp. LL172 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.14 | L-Mannose | only reverse direction described. At equilibrium the production of L-mannose from L-fructose is 30% | Pseudomonas sp. | L-Fructose | - |
? | |
5.3.1.14 | L-Mannose | only reverse direction described. At equilibrium the production of L-mannose from L-fructose is 30% | Pseudomonas sp. LL172 | L-Fructose | - |
? | |
5.3.1.14 | L-Rhamnose | - |
Pseudomonas sp. | L-Rhamnulose | - |
? | |
5.3.1.14 | L-Rhamnose | - |
Pseudomonas sp. LL172 | L-Rhamnulose | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 60 | - |
immobilized enzyme | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 60 | - |
10 min stable, glycine-NaOH buffer, immobilized enzyme | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 5 | 11 | immobilized enzyme | Pseudomonas sp. |
5.3.1.14 | 9 | - |
free enzyme | Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 5 | 11 | pH-range of optimal activity, immobilized enzyme | Pseudomonas sp. |
5.3.1.14 | 7 | 11 | pH 7: about 50% of maximal activity, pH 11: about 40% of maximal activity, free enzyme | Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 5 | 11 | 4°C, 24 h, immobilized enzyme remains stable | Pseudomonas sp. |