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Literature summary extracted from

  • Sano, M.; Hayakawa, K.; Kato, I.
    An enzyme releasing lacto-N-biose from oligosaccharides (1992), Proc. Natl. Acad. Sci. USA, 89, 8512-8516.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.140 analysis the enzyme may be useful in identifying type 1 structure in glycoconjugates and to distinguish type 1 oligosaccharides from type 2 oligosaccharides Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.140 Streptomyces sp.
-
-
-
3.2.1.140 Streptomyces sp. 142
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.140 lacto-N-tetraose + H2O
-
 Streptomyces sp. lactose + lacto-N-biose
-
 ?
3.2.1.140 lacto-N-tetraose + H2O
-
 Streptomyces sp. 142 lactose + lacto-N-biose
-
 ?
3.2.1.140 additional information no reaction with lacto-N-neotetraose, lacto-N-triose, sialyl lacto-N-tetraose, lacto-N-fucopentaose I, II, or III, asialo-GM1 tetrasaccharide, poly-N-acetyllactosamine Streptomyces sp. ?
-
 ?
3.2.1.140 additional information no reaction with lacto-N-neotetraose, lacto-N-triose, sialyl lacto-N-tetraose, lacto-N-fucopentaose I, II, or III, asialo-GM1 tetrasaccharide, poly-N-acetyllactosamine Streptomyces sp. 142 ?
-
 ?
3.2.1.140 p-nitrophenyl-lacto-N-bioside + H2O
-
 Streptomyces sp. p-nitrophenol + lacto-N-biose
-
 ?
3.2.1.140 p-nitrophenyl-lacto-N-bioside + H2O
-
 Streptomyces sp. 142 p-nitrophenol + lacto-N-biose
-
 ?
3.2.1.140 pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
-
 Streptomyces sp. lacto-N-biose + ? from N-acetyllactosamine type of triantennary sugar chain with type 1 structure and N-avcetyllactosamine type of biantennary sugar chain is produced ?
3.2.1.140 pyridylamine-oligosaccharides which have type 1 chains at the nonreducing terminus + H2O
-
 Streptomyces sp. 142 lacto-N-biose + ? from N-acetyllactosamine type of triantennary sugar chain with type 1 structure and N-avcetyllactosamine type of biantennary sugar chain is produced ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.140 50
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.140 50
-
 30 min, 20% loss of activity Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.140 5.5
-
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.140 4 7 4°C, 16 h, stable Streptomyces sp.