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Literature summary extracted from

  • Uchida, H.; Nanri, T.; Kawabata, Y.; Kusakabe, I.; Murakami, K.
    Purification and characterization of intracellular alpha-glucuronidase from Aspergillus niger 5-16 (1992), Biosci. Biotechnol. Biochem., 56, 1608-1615.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.139 Ag+
-
 Aspergillus niger
3.2.1.139 Al3+
-
 Aspergillus niger
3.2.1.139 Fe2+
-
 Aspergillus niger
3.2.1.139 Fe3+
-
 Aspergillus niger
3.2.1.139 Hg2+
-
 Aspergillus niger
3.2.1.139 Monoiodoacetic acid
-
 Aspergillus niger
3.2.1.139 N-bromosuccinimide
-
 Aspergillus niger
3.2.1.139 SDS
-
 Aspergillus niger
3.2.1.139 Sn2+
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.139 0.37
-
 4-O-Methyl-glucuronosyl-xylotriose enzyme form CM-I Aspergillus niger
3.2.1.139 0.47
-
 4-O-Methyl-glucuronosyl-xylotriose enzyme form CM-II Aspergillus niger
3.2.1.139 0.77
-
 glucuronosyl-xylotriose enzyme form CM-I Aspergillus niger
3.2.1.139 0.82
-
 glucuronosyl-xylotriose enzyme form CM-II Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.139 intracellular
-
 Aspergillus niger 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.139 130000
-
 1 * 130000, SDS-PAGE Aspergillus niger
3.2.1.139 150000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.139 Aspergillus niger
-
-
-
3.2.1.139 Aspergillus niger 5-16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.139 2 enzym forms: CM-I and CM-II Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.139 additional information
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.139 4-O-methyl-glucuronosyl-xylotriose + H2O
-
 Aspergillus niger 4-O-methyl-glucuronate + xylotriose
-
 ?
3.2.1.139 4-O-methyl-glucuronosyl-xylotriose + H2O
-
 Aspergillus niger 5-16 4-O-methyl-glucuronate + xylotriose
-
 ?
3.2.1.139 glucuronosyl-xylotriose + H2O
-
 Aspergillus niger glucuronate + xylotriose
-
 ?
3.2.1.139 glucuronosyl-xylotriose + H2O
-
 Aspergillus niger 5-16 glucuronate + xylotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.139 monomer 1 * 130000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.139 60
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.139 50
-
 pH 4.5-7.0, 1 h, stable Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.139 4.8
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.139 4 5.6 about 50% of maximal activity at pH 4.5 and 5.6 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.139 4.5 7 50°C, 1 h, stable Aspergillus niger