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Literature summary extracted from

  • Marcinowski, S.; Grisebach, H.
    Enzymology of lignification. Cell-wall bound beta-glucosidase for coniferin from spruce (Picea abies) seedlings (1978), Eur. J. Biochem., 87, 37-44.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.126 0.38
-
 coniferin roots Picea abies
3.2.1.126 0.6
-
 Picein 2-nitrophenyl beta-D-glucoside, 2-nitrophenyl beta-D-galactoside, hypocotyls Picea abies
3.2.1.126 0.7
-
 4-nitrophenyl beta-D-glucoside hypocotyls Picea abies
3.2.1.126 1
-
 4-methylumbelliferyl beta-D-glucoside hypocotyls Picea abies
3.2.1.126 1.3
-
 coniferin hypocotyls Picea abies
3.2.1.126 1.8
-
 syringin hypocotyls Picea abies
3.2.1.126 6.3
-
 Picein hypocotyls Picea abies

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.126 cell wall
-
 Picea abies 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.126 57000
-
 1 * 57000, Picea abies, SDS-PAGE Picea abies
3.2.1.126 58570
-
 Picea abies, sedimentation equilibrium method Picea abies

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.126 coniferin + H2O Picea abies plant cell wall enzyme, involved in lignin biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.126 Picea abies
-
 spruce
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.126 hypocotyls Picea abies

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.126 hypocotyl
-
 Picea abies
-
3.2.1.126 root
-
 Picea abies
-
3.2.1.126 seedling tissue distribution Picea abies
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.126 4.5
-
 hypocotyls Picea abies

Storage Stability

EC Number Storage Stability Organism
3.2.1.126 -20°C, crude cell wall preparation, frozen with liquid nitrogen, at least 3 months Picea abies
3.2.1.126 4°C, in 0.025 M McIlvaine buffer, pH 5, 0.1 M NaCl, 0.02% NaN3, less than 10% loss of activity within 15 days Picea abies

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.126 2-nitrophenyl beta-D-galactoside + H2O reaction at 16% (isozyme I) or 13% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 2-nitrophenol + D-galactose
-
 ?
3.2.1.126 2-nitrophenyl beta-D-glucoside + H2O best substrate Picea abies 2-nitrophenol + D-glucose
-
 ?
3.2.1.126 2-nitrophenyl beta-D-xyloside + H2O poor substrate Picea abies 2-nitrophenol + D-xylose
-
 ?
3.2.1.126 4-coumaryl beta-D-glucoside + H2O reaction at 25% (isozyme II) or 4% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies coumaric acid + D-glucose
-
 ?
3.2.1.126 4-methylumbelliferyl beta-D-glucoside + H2O reaction at 79% (isozyme II) or 51% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 4-methylumbelliferone + D-glucose
-
 ?
3.2.1.126 4-nitrophenyl beta-D-galactoside + H2O poor substrate, not Picea abies 4-nitrophenol + D-galactose
-
 ?
3.2.1.126 4-nitrophenyl beta-D-glucoside + H2O reaction at 56% (isozyme II) or 8% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies 4-nitrophenol + D-glucose
-
 ?
3.2.1.126 coniferin + H2O reaction at 52% (isozyme I) or 38% (isozyme II) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies coniferol + D-glucose
-
 ?
3.2.1.126 coniferin + H2O plant cell wall enzyme, involved in lignin biosynthesis Picea abies ?
-
 ?
3.2.1.126 feruloyl glucoside + H2O poor substrate Picea abies ferulic acid + glucose
-
 ?
3.2.1.126 picein + H2O reaction at 50% (isozyme II) or 12% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies ?
-
 ?
3.2.1.126 salicin + H2O reaction at 25% (isozyme II) or 2% (isozyme I) the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies ?
-
 ?
3.2.1.126 syringin + H2O reaction at about 30% the rate of 2-nitrophenyl beta-D-glucoside hydrolysis Picea abies D-glucose + sinapyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.126 monomer 1 * 57000, Picea abies, SDS-PAGE Picea abies

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.126 30
-
 assay at Picea abies
3.2.1.126 40 45
-
 Picea abies

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.126 additional information
-
 pI: 10 (isozyme I), pI: 10.3 (isozyme II) Picea abies
3.2.1.126 4.5 5.5 hypocotyls Picea abies

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.126 3.5 6.5 about half-maximal activity at pH 3.5 and 6.5, hypocotyls Picea abies