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Literature summary extracted from

  • De Souza, M.L.; Sadowsky, M.J.; Wackett, L.P.
    Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization (1996), J. Bacteriol., 178, 4894-4900.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.8.1.8 agriculture degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.8 0.149
-
 Atrazine
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.8.1.8 additional information no metal ions detectable with the enzym expressed in E. coli Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.8 245000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.8 atrazine + H2O Pseudomonas sp.
-
 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.8 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.8
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.8 4.5
-
 purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.8 atrazine + H2O
-
 Pseudomonas sp. 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?
3.8.1.8 desethylatrazine + H2O
-
 Pseudomonas sp. 2-hydroxy-4-amino-6-(isopropylamino)-1,3,5-triazine + HCl
-
 ?
3.8.1.8 simazine + H2O
-
 Pseudomonas sp. 4,6-diethylamino-1,3,5-triazine + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.8 pentamer 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence Pseudomonas sp.
3.8.1.8 tetramer 4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.8.1.8 AtzA
-
-

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8.1.8 11
-
 Atrazine
-
 Pseudomonas sp.