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Literature summary extracted from

  • Seltzer, S.
    Maleylacetoacetate cis-trans isomerase (1989), Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York, 3, 733-751.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.2.1.2 GSH required as coenzyme Vibrio sp.
5.2.1.2 GSH other thiols are inactive Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.2 GSSG
-
 Vibrio sp.
5.2.1.2 N-Hexylmaleamic acid noncompetitive towards GSH and maleylacetone Vibrio sp.
5.2.1.2 NEM
-
 Vibrio sp.
5.2.1.2 S-Glutathioyl-N-hexylmaleamate competitive towards GSH, uncompetitive towards maleylacetone Vibrio sp.
5.2.1.2 S-Methylglutathione
-
 Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2.1.2 0.8
-
 maleylacetone
-
 Vibrio sp.
5.2.1.2 1.9
-
 6-Oxo-2(E),4(Z)-heptanedienoic acid
-
 Vibrio sp.
5.2.1.2 2.9
-
 trans-beta-Acetylacrylate
-
 Vibrio sp.
5.2.1.2 3
-
 cis-beta-Acetylacrylate
-
 Vibrio sp.
5.2.1.2 3.2
-
 6-Oxo-2(Z),4(E)-heptanedienoic acid
-
 Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.2.1.2 16000
-
 x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea Vibrio sp.
5.2.1.2 35000
-
 gel filtration Vibrio sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.2.1.2 Maleylacetoacetate Vibrio sp. one of the enzymes responsible for the oxidative metabolism of aromatic amino acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.2 Mammalia
-
-
-
5.2.1.2 Vibrio sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.2.1.2 liver
-
 Mammalia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.2 6-Oxo-2(E),4(Z)-heptanedienoic acid
-
 Vibrio sp. 6-Oxo-2(Z),4(E)-heptanedienoic acid
-
 ?
5.2.1.2 cis-beta-Acetylacrylate
-
 Vibrio sp. trans-beta-Acetylacrylate
-
 ?
5.2.1.2 Maleylacetoacetate
-
 Vibrio sp. Fumarylacetoacetate
-
 ?
5.2.1.2 Maleylacetoacetate one of the enzymes responsible for the oxidative metabolism of aromatic amino acids Vibrio sp. ?
-
 ?
5.2.1.2 maleylacetone
-
 Vibrio sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.2.1.2 ? x * 35000, SDS-PAGE Vibrio sp.
5.2.1.2 ? x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea Vibrio sp.