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Literature summary extracted from

  • Omori, T.; Sugimura, K.; Ishigooka, H.; Minoda, Y.
    Purification and some propertiees of a 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolyzing enzyme from Pseudomonas cruciviae S93 B1 involved in the degradation of biphenyl (1986), Agric. Biol. Chem., 50, 931-937.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.8 29000
-
x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme Pseudomonas cruciviae
3.7.1.8 160000
-
gel filtration Pseudomonas cruciviae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.8 additional information Pseudomonas cruciviae enzyme is involved in the degradation of biphenyl ?
-
?
3.7.1.8 additional information Pseudomonas cruciviae S93 B1 enzyme is involved in the degradation of biphenyl ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.8 Pseudomonas cruciviae
-
S93 B1
-
3.7.1.8 Pseudomonas cruciviae S93 B1
-
S93 B1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.8
-
Pseudomonas cruciviae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.8 51.39
-
-
Pseudomonas cruciviae

Storage Stability

EC Number Storage Stability Organism
3.7.1.8 4°C, pH 7.4, 10% loss of activity after 1 month Pseudomonas cruciviae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.8 3-isopropylcatechol + H2O
-
Pseudomonas cruciviae ?
-
?
3.7.1.8 3-isopropylcatechol + H2O
-
Pseudomonas cruciviae S93 B1 ?
-
?
3.7.1.8 3-methylcatechol + H2O
-
Pseudomonas cruciviae ?
-
?
3.7.1.8 3-methylcatechol + H2O
-
Pseudomonas cruciviae S93 B1 ?
-
?
3.7.1.8 biphenyl-2,3-diol + H2O
-
Pseudomonas cruciviae ?
-
?
3.7.1.8 biphenyl-2,3-diol + H2O
-
Pseudomonas cruciviae S93 B1 ?
-
?
3.7.1.8 catechol + H2O
-
Pseudomonas cruciviae ?
-
?
3.7.1.8 catechol + H2O
-
Pseudomonas cruciviae S93 B1 ?
-
?
3.7.1.8 additional information enzyme is involved in the degradation of biphenyl Pseudomonas cruciviae ?
-
?
3.7.1.8 additional information enzyme is involved in the degradation of biphenyl Pseudomonas cruciviae S93 B1 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.7.1.8 ? x * 29000, SDS-PAGE of mercaptoethanol denatured enzyme Pseudomonas cruciviae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.7.1.8 20
-
1 h, stable Pseudomonas cruciviae
3.7.1.8 60
-
pH 7.4, 5 min, 75% loss of activity Pseudomonas cruciviae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.8 7.4
-
-
Pseudomonas cruciviae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.7.1.8 6.5 8.7 pH 6.5: about 65% of maximal activity, pH 8.7: about 25% of maximal activity Pseudomonas cruciviae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.7.1.8 9 11 25°C, 6 h, stable Pseudomonas cruciviae