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Literature summary extracted from
- Purification and characterization of the proton translocating plasma membrane ATPase of red beet storage tissue (1990), Biochim. Biophys. Acta, 1039, 241-252.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | lysophosphatidylcholine | stimulates | Beta vulgaris |  |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 7.1.2.1 | two-dimensional crystallization of the reconstituted enzyme | Beta vulgaris |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | Dicyclohexylcarbodiimide | - |
Beta vulgaris | |
| 7.1.2.1 | Na2MO4 | weak | Beta vulgaris | |
| 7.1.2.1 | vanadate | Na3VO4, non-competitive | Beta vulgaris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.2.1 | 1.5 | - |
ATP | - |
Beta vulgaris | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.2.1 | plasma membrane | - |
Beta vulgaris | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.2.1 | K+ | KCl stimulates | Beta vulgaris | |
| 7.1.2.1 | Mg2+ | required | Beta vulgaris | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 100000 | - |
x * 100000, SDS-PAGE | Beta vulgaris |
| 7.1.2.1 | 600000 | - |
gel filtration | Beta vulgaris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.2.1 | Beta vulgaris | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 7.1.2.1 | side-chain modification | contains 80 mol of phospholipid per mol of 100000 Da ATPase, 60% phosphatidylcholine, 30% phosphatidylethanolamine, 5% lysophosphatidylcholine | Beta vulgaris |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.2.1 | - |
Beta vulgaris |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.2.1 | storage tissue | - |
Beta vulgaris | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 25.4 | - |
- |
Beta vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.2.1 | ATP + H2O + 4 H+[side 1] | ATP in form of MgATP2- | Beta vulgaris | ADP + phosphate + 4 H+[side 2] | - |
? | |
| 7.1.2.1 | ATP + H2O + H+/in | - |
Beta vulgaris | ADP + phosphate + H+/out | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.2.1 | ? | x * 100000, SDS-PAGE | Beta vulgaris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 6.5 | - |
- |
Beta vulgaris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.2.1 | 5.4 | 7 | about 45% of maximal activity at pH 5.4 and at pH 7.0 | Beta vulgaris |
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