Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Krishnan, N.; Appaji Rao, N.
    Studies on nucleotide pyrophosphatase I. Partial purification and properties of a sheep liver enzyme that catalyses the hydrolysis of dinucleotides (1972), Arch. Biochem. Biophys., 149, 336-348.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.9 phosphate enhanced release of FMN at 0.01 mM, increases Vmax Ovis aries aries

General Stability

EC Number General Stability Organism
3.6.1.9 complete inactivation if repeated freezed and thawed Ovis aries aries

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 5'-ADP complete inhibition at 1 mM Ovis aries aries
3.6.1.9 5'-AMP sigmoid type of inhibition, phosphate abolishes sigmoidal response Ovis aries aries
3.6.1.9 ATP potent inhibition at 0.1 mM Ovis aries aries
3.6.1.9 CTP potent inhibition at 0.1 mM Ovis aries aries
3.6.1.9 diphosphate complete inhibition above 0.1 mM Ovis aries aries
3.6.1.9 EDTA
-
 Ovis aries aries
3.6.1.9 FAD+ 50% inhibition at 1-2 mM Ovis aries aries
3.6.1.9 FeSO4 42% inhibition at 1 mM in the presence of 0.1 mM o-phenanthroline Ovis aries aries
3.6.1.9 GMP complete inhibition at 1 mM Ovis aries aries
3.6.1.9 GSH 40% inhibition at 1 mM Ovis aries aries
3.6.1.9 GSSG 25% inhibition at 1 mM Ovis aries aries
3.6.1.9 ITP potent inhibition at 0.1 mM Ovis aries aries
3.6.1.9 o-phenanthroline 60-90% inhibition at 0.1 mM, reversal by FeSO4 Ovis aries aries
3.6.1.9 p-hydroxymercuribenzoate 60% inhibition at 1 mM, reversal by GSH Ovis aries aries
3.6.1.9 TTP 70% inhibition at 1 mM Ovis aries aries
3.6.1.9 UTP potent inhibition at 0.1 mM Ovis aries aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.133
-
 FAD with and without phosphate which abolishes negative cooperative interaction Ovis aries aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.9 FAD + H2O Ovis aries aries
-
 FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O Ovis aries aries
-
 NMN + AMP
-
 ?
3.6.1.9 NADP+ + H2O Ovis aries aries
-
 NMN + adenosine 2',5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Ovis aries aries
-
 sheep
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 partial Ovis aries aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 liver
-
 Ovis aries aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 107
-
-
 Ovis aries aries

Storage Stability

EC Number Storage Stability Organism
3.6.1.9 5°C, 80% of activity remains after 16 h Ovis aries aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 FAD + H2O
-
 Ovis aries aries FMN + AMP
-
 ?
3.6.1.9 NAD+ + H2O
-
 Ovis aries aries NMN + AMP
-
 ?
3.6.1.9 NADP+ + H2O
-
 Ovis aries aries NMN + adenosine 2',5'-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.9 30 36 first optimum Ovis aries aries
3.6.1.9 42
-
 in the presence of phosphate Ovis aries aries
3.6.1.9 60
-
 second optimum Ovis aries aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.9 9.7
-
-
 Ovis aries aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.9 1 2 FAD+
-
 Ovis aries aries