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Literature summary extracted from

  • Chiti, F.; van Nuland, N.A.J.; Taddei, N.; Magherini, F.; Stefani, M.; Ramponi, G.; Dobson, C.M.
    Conformational stability of muscle acylphosphatase: the role of temperature, denaturant concentration, and pH (1998), Biochemistry, 37, 1447-1455.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.7 highest stability against urea is at pH 9.5 Homo sapiens
3.6.1.7 muscular enzyme, sensitive to urea: urea denaturation Homo sapiens
3.6.1.7 no cold denaturation Homo sapiens
3.6.1.7 studies on conformational stability of muscle acylphosphatase Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.7 an acylphosphate + H2O = a carboxylate + phosphate thermodynamic analysis, studies and data Homo sapiens
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.6.1.7 renaturation after heat denaturation Homo sapiens
3.6.1.7 renaturation after treatment with urea Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Homo sapiens 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Homo sapiens acetate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens carboxylate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Homo sapiens carbamate + phosphate
-
 ?
3.6.1.7 succinoyl phosphate + H2O
-
 Homo sapiens succinate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.7 More two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.7 additional information
-
 no cold denaturation Homo sapiens
3.6.1.7 additional information
-
 increase of urea concentration causes reduction of the thermoresistance of the protein Homo sapiens
3.6.1.7 additional information
-
 studies on conformational stability of muscle acylphosphatase Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 additional information
-
 muscle, pI: 11.4 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.7 3.5 6.5 pH range of maximum catalytic activity Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.7 additional information
-
 studies on conformational stability of muscle acylphosphatase Homo sapiens
3.6.1.7 2.5 11 stable between, enzyme is in its native state between, outside this pH range, stability of enzyme collapses abruptly, most probably due to excess of positive or negative charges present at low and high pH Homo sapiens
3.6.1.7 3.5 6.5 highest conformational stability between Homo sapiens
3.6.1.7 5.5
-
 maximum stability near pH 5.5 Homo sapiens
3.6.1.7 9.5
-
 highest stability against urea at pH 9.5 Homo sapiens