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Literature summary extracted from

  • Stefani, M.; Degl'Innocenti, D.; Berti, A.; Cappugi, G.; Manao, G.; Camici, G.; Ramponi, G.
    Purification and characterization of acylphosphatase erythrocyte isoenzyme from turkey muscle (1990), J. Protein Chem., 9, 633-640.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.6.1.7 enzyme very sensitive to 8 M urea Meleagris gallopavo

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.7 Cl- muscular isoenzyme, Ki: 40.0 mM, erythrocyte isoenzyme, Ki: 31.0 mM Meleagris gallopavo
3.6.1.7 Phenylglyoxal 6 mM: inhibition, inhibition partially removed by 35 mM phosphate Meleagris gallopavo
3.6.1.7 phosphate muscular isoenzyme, Ki: 2.8 mM, erythrocyte isoenzyme, Ki: 0.68 mM Meleagris gallopavo
3.6.1.7 Urea 8 M: 100% inhibition; 8 M: 100% reversibel inhibition, 60% reactivation in the absence of urea Meleagris gallopavo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.7 additional information
-
 additional information kinetic data Meleagris gallopavo
3.6.1.7 0.18
-
 benzoyl phosphate erythrocyte isoenzyme Meleagris gallopavo
3.6.1.7 1
-
 benzoyl phosphate muscular isoenzyme Meleagris gallopavo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.7 12750
-
 SDS-PAGE Meleagris gallopavo
3.6.1.7 12750
-
 erythrocyte isoenzyme Meleagris gallopavo
3.6.1.7 13400
-
 muscular isoenzyme Meleagris gallopavo

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Meleagris gallopavo
-
 turkey
-
3.6.1.7 Meleagris gallopavo
-
 two isoenzymes: muscular isoenzyme, IM, and erythrocyte isoenzyme, IE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7 muscle, erythrocyte isoenzyme Meleagris gallopavo

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.7 an acylphosphate + H2O = a carboxylate + phosphate thermodynamic data Meleagris gallopavo
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 muscle skeletal muscle Meleagris gallopavo
-
3.6.1.7 muscle erythrocyte isoenzyme Meleagris gallopavo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.7 3500
-
 muscular isoenzyme Meleagris gallopavo
3.6.1.7 9000
-
 erythrocyte isoenzyme Meleagris gallopavo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Meleagris gallopavo 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Meleagris gallopavo carboxylate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Meleagris gallopavo benzoate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.7 monomer erythrocyte isoenzyme, 1 * 12750, SDS-PAGE Meleagris gallopavo

Synonyms

EC Number Synonyms Comment Organism
3.6.1.7 More two isoenzymes in all tissues, organs, and cell types checked: 1. muscular isoenzyme, IM, and 2. erythrocyte isoenzyme, IE, on the basis of the cell-type in which they are mostly represented and from which they are purified for the first time Meleagris gallopavo

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.7 additional information
-
 enzyme is thermostable Meleagris gallopavo
3.6.1.7 additional information
-
 enzyme is thermostable, thermostability is pH-dependent, turkey enzyme is less unstable at higher pH values than horse enzyme Meleagris gallopavo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 additional information
-
 erythrocyte enzyme is a basic protein Meleagris gallopavo
3.6.1.7 5.3
-
 muscular and erythrocyte isoenzyme Meleagris gallopavo