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Literature summary extracted from

  • Shiokawa, H.; Noda, L.
    Isolation and crystallization of acyl phosphatase from rabbit muscle (1970), J. Biol. Chem., 245, 669-673.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.7
-
 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.7 23500
-
 sedimentation equilibrium Oryctolagus cuniculus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.6.1.7 Acetone 70% v/v, stable at -10°C for 3 h Oryctolagus cuniculus
3.6.1.7 Ethanol unstable to Oryctolagus cuniculus
3.6.1.7 Methanol unstable to Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.7 860
-
 muscle Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.6.1.7 -10°C, crystalline enzyme, 42% ammonium sulfate, stable for long periods, 1 year with no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 acetyl phosphate + H2O
-
 Oryctolagus cuniculus acetate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Oryctolagus cuniculus carboxylate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 5.4
-
 muscle, assay at Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.7 additional information
-
 stable at acidic and neutral pH, somewhat unstable at alkaline pH Oryctolagus cuniculus
3.6.1.7 3 5.4 stable 12 h , 37°C Oryctolagus cuniculus
3.6.1.7 9
-
 1 h, 2°C: 30% loss of activity Oryctolagus cuniculus