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Literature summary extracted from

  • Liguri, G.; Camici, G.; Manao, G.; Cappugi, G.; Nassi, P.; Modesti, A.; Ramponi, G.
    A new acylphosphatase isoenzyme from human erythrocytes: purification, characterization, and primary structure (1986), Biochemistry, 25, 8089-8094.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.7 2,3-diphosphoglycerate competitive inhibition, Ki erythrocyte enzyme: 0.41 mM, Ki skeletal muscle enzyme: 3.74 mM Homo sapiens
3.6.1.7 Cl- competitive inhibition, Ki erythrocyte enzyme: 51.70 mM, Ki skeletal muscle enzyme: 42.10 mM Homo sapiens
3.6.1.7 phosphate competitive inhibition, Ki erythrocyte enzyme: 0.30 mM, Ki skeletal muscle enzyme: 2.72 mM Homo sapiens
3.6.1.7 phosphoenolpyruvate competitive inhibition, Ki erythrocyte enzyme: 0.38 mM, Ki skeletal muscle enzyme: 2.23 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.7 additional information
-
 additional information kinetic characterization, kinetic data Homo sapiens
3.6.1.7 0.15
-
 benzoyl phosphate erythrocyte enzyme Homo sapiens
3.6.1.7 0.55
-
 1,3-diphosphoglycerate erythrocyte enzyme Homo sapiens
3.6.1.7 0.62
-
 1,3-diphosphoglycerate skeletal muscle enzyme Homo sapiens
3.6.1.7 0.81
-
 benzoyl phosphate skeletal muscle enzyme Homo sapiens
3.6.1.7 2.9
-
 acetyl phosphate erythrocyte enzyme Homo sapiens
3.6.1.7 4.2
-
 Carbamoyl phosphate skeletal muscle enzyme Homo sapiens
3.6.1.7 5.42
-
 Carbamoyl phosphate erythrocyte enzyme Homo sapiens
3.6.1.7 6.2
-
 acetyl phosphate skeletal muscle enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.7 11170
-
 erythrocyte enzyme, calculated from amino acid sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.7 Homo sapiens
-
 human
-
3.6.1.7 Homo sapiens
-
 two different isoenzymes: skeletal muscle and erythrocyte enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.7 erythrocyte isoenzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.7 erythrocyte erythrocyte isoenzyme Homo sapiens
-
3.6.1.7 muscle skeletal muscle Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.7 7500
-
 erythrocyte isoenzyme, substrate benzoyl phosphate, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.7 1,3-diphosphoglycerate + H2O
-
 Homo sapiens 3-phosphoglycerate + phosphate
-
 ?
3.6.1.7 acetyl phosphate + H2O
-
 Homo sapiens acetate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O erythrocyte isoenzyme shows hydrolytic activity on acyl phosphates with higher affinity than the muscle enzyme Homo sapiens carboxylate + phosphate
-
 ?
3.6.1.7 acylphosphate + H2O specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens carboxylate + phosphate
-
 ?
3.6.1.7 benzoyl phosphate + H2O
-
 Homo sapiens benzoate + phosphate
-
 ?
3.6.1.7 carbamoyl phosphate + H2O
-
 Homo sapiens carbamate + phosphate
-
 ?
3.6.1.7 additional information substrate specificity Homo sapiens ?
-
 ?
3.6.1.7 additional information specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates Homo sapiens ?
-
 ?
3.6.1.7 additional information specific phosphomonohydrolase activity Homo sapiens ?
-
 ?
3.6.1.7 p-nitrobenzoyl phosphate + H2O
-
 Homo sapiens p-nitrobenzoate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.7 25
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.7 37
-
 90% loss of activity after 10 min at, pH range 5.3-7.4 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.7 5.3
-
 assay at Homo sapiens
3.6.1.7 5.3
-
 skeletal muscle enzyme Homo sapiens
3.6.1.7 5.5
-
 erythrocyte enzyme Homo sapiens