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Literature summary extracted from

  • Mukhopadhyay, B.; Purwantini, E.; Daniels, L.
    Effect of methanogenic substrates on coenzyme f420-dependent N5,N10-methylene-H4MPT dehydrogenase, N5,N10-methenyl-H4MPT cyclohydrolase and F420-reducing hydrogenase activities in Methanosarcina barkeri (1993), Arch. Microbiol., 159, 141-146.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + coenzyme F420 Methanosarcina barkeri enzyme of methanogenesis pathway reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 Methanosarcina barkeri Fusaro / DSM 804 enzyme of methanogenesis pathway reduced coenzyme F420
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanosarcina barkeri in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold ?
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanosarcina barkeri Fusaro / DSM 804 in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanosarcina barkeri
-
activity inducible by choice of substrate
-
1.12.98.1 Methanosarcina barkeri Fusaro / DSM 804
-
activity inducible by choice of substrate
-
3.5.4.27 Methanosarcina barkeri
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 0.34
-
growth with H2 and CO2, enzyme activity depends on growth substrate Methanosarcina barkeri
1.12.98.1 0.437
-
growth on methanol, enzyme activity depends on growth substrate Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + coenzyme F420
-
Methanosarcina barkeri reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanosarcina barkeri reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420
-
Methanosarcina barkeri Fusaro / DSM 804 reduced coenzyme F420
-
?
1.12.98.1 H2 + coenzyme F420 enzyme of methanogenesis pathway Methanosarcina barkeri Fusaro / DSM 804 reduced coenzyme F420
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
Methanosarcina barkeri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
Methanosarcina barkeri Fusaro / DSM 804 N5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold Methanosarcina barkeri ?
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O in acetate-grown cells the cyclohydrolase level is lowered 2 to 3fold, in cultures growing on acetate supplemented with methanol or H2CO cyclohydrolase activity level increases 3 to 4fold Methanosarcina barkeri Fusaro / DSM 804 ?
-
?