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Literature summary extracted from

  • Vaupel, M.; Vorholt, J.A.; Thauer, R.K.
    Overproduction and one-step purification of the N5,N10-methenyltetrahydromethanopterin cyclohydrolase (Mch) from the hyperthermophilic Methanopyrus kandleri (1998), Extremophiles, 2, 15-22.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.27 expression in Escherichia coli Methanopyrus kandleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.27 0.03
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin recombinant enzyme Methanopyrus kandleri
3.5.4.27 0.04
-
 N5,N10-methenyl-5,6,7,8-tetrahydromethanopterin
-
 Methanopyrus kandleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.27 41000
-
 ? * 41000, SDS-PAGE Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanosarcina barkeri
-
 ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanopyrus kandleri
-
 ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O Methanothermobacter thermautotrophicus enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.27 Methanocaldococcus jannaschii
-
-
-
3.5.4.27 Methanopyrus kandleri
-
-
-
3.5.4.27 Methanosarcina barkeri
-
-
-
3.5.4.27 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.27
-
 Methanopyrus kandleri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.27 5410
-
-
 Methanopyrus kandleri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanosarcina barkeri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanothermobacter thermautotrophicus N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanocaldococcus jannaschii N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanopyrus kandleri N5-formyl-5,6,7,8-tetrahydromethanopterin
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanosarcina barkeri ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
 Methanopyrus kandleri ?
-
 ?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O enzyme is involved in methanogenesis from CO2 and H2, which represents the energy metabolism Methanothermobacter thermautotrophicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.27 ? ? * 41000, SDS-PAGE Methanopyrus kandleri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.27 90
-
 pH 8.0, 40 mM K2HPO4, stable up to Methanopyrus kandleri