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Literature summary extracted from

  • Ogawa, J.; Min Kim, J.; Nirdnoy, W.; Amano, Y.; Yamada, H.; Shimizu, S.
    Purification and characterization of an ATP-dependent amidohydrolase, N-methylhydantoin amidohydrolase, from Pseudomonas putida 77 (1995), Eur. J. Biochem., 229, 184-190.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.14 addition of small portions of solid ammonium sulfate to the concentrated enzyme solution Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.14 5,5'-dithio-bis(2-nitrobenzoic acid) 0.1 mM Pseudomonas putida
3.5.2.14 8-hydroxyquinoline 1 mM Pseudomonas putida
3.5.2.14 Ag2+ 1 mM Pseudomonas putida
3.5.2.14 Cd2+ 1 mM Pseudomonas putida
3.5.2.14 Cu2+ 1 mM Pseudomonas putida
3.5.2.14 Fe2+ 1 mM Pseudomonas putida
3.5.2.14 Hg2+ 0.1 mM Pseudomonas putida
3.5.2.14 hydroxylamine 1 mM Pseudomonas putida
3.5.2.14 N-Methylmaleimide 1 mM Pseudomonas putida
3.5.2.14 p-chloromercuribenzoate 0.1 mM Pseudomonas putida
3.5.2.14 phenylhydrazine 1 mM Pseudomonas putida
3.5.2.14 Semicarbazide 1 mM Pseudomonas putida
3.5.2.14 Zn2+ 1 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.14 0.032
-
 N-methylhydantoin
-
 Pseudomonas putida
3.5.2.14 0.07
-
 glutarimide
-
 Pseudomonas putida
3.5.2.14 0.083
-
 dihydrouracil
-
 Pseudomonas putida
3.5.2.14 0.11
-
 delta-valerolactam
-
 Pseudomonas putida
3.5.2.14 0.13
-
 succimide
-
 Pseudomonas putida
3.5.2.14 0.75
-
 2-Pyrrolidone
-
 Pseudomonas putida
3.5.2.14 1.7
-
 hydantoin
-
 Pseudomonas putida
3.5.2.14 2
-
 Dihydrothymine
-
 Pseudomonas putida
3.5.2.14 2
-
 D,L-5-methylhydantoin
-
 Pseudomonas putida
3.5.2.14 6.6
-
 Uracil
-
 Pseudomonas putida
3.5.2.14 10
-
 2,4-Thiazolidinedione
-
 Pseudomonas putida
3.5.2.14 16
-
 thymine
-
 Pseudomonas putida
3.5.2.14 18
-
 2-Oxazolidone
-
 Pseudomonas putida
3.5.2.14 40
-
 2-Imidazolidone
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.14 K+ monovalent cations Pseudomonas putida
3.5.2.14 Mg2+ divalent cations Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.14 70000
-
 alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida
3.5.2.14 80000
-
 alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida
3.5.2.14 300000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.14 ATP + N-methylimidazolidine-2,4-dione + H2O Pseudomonas putida
-
 ADP + phosphate + N-carbamoylsarcosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.14 Pseudomonas putida
-
 77
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.14
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.14 ATP + 2,4-thiazolidinedione + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + 2-imidazolidone + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + 2-oxazolidone + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + 2-pyrrolidone + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + D,L-5-methylhydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + delta-valerolactam + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + dihydrothymine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + dihydrouracil + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + glutarimide + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + hydantoin + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + N-methylimidazolidine-2,4-dione + H2O
-
 Pseudomonas putida ADP + phosphate + N-carbamoylsarcosine
-
 ?
3.5.2.14 ATP + succimide + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + thymine + H2O
-
 Pseudomonas putida ?
-
 ?
3.5.2.14 ATP + uracil + H2O
-
 Pseudomonas putida ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.14 tetramer alpha2beta2 2 * 70000 + 2 * 80000, SDS-PAGE Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.14 20 50
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.14 6 10
-
 Pseudomonas putida