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Literature summary extracted from

  • Wintzerith, M.; Dierich, A.; Mandel, P.
    Purification and characterization of a nicotinamide deamidase released into the growth medium of neuroblastoma in vitro (1980), Biochim. Biophys. Acta, 613, 191-202.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.19 Serum albumin 0.02%, 9% activation Mus musculus

General Stability

EC Number General Stability Organism
3.5.1.19 freezing and defreezing inactivates Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.19 2-mercaptoethanol
-
 Mus musculus
3.5.1.19 Ag+
-
 Mus musculus
3.5.1.19 Cu2+
-
 Mus musculus
3.5.1.19 Cys
-
 Mus musculus
3.5.1.19 dithiothreitol
-
 Mus musculus
3.5.1.19 EDTA 0.02 mM, 46% inhibition Mus musculus
3.5.1.19 Fe2+
-
 Mus musculus
3.5.1.19 Hg2+
-
 Mus musculus
3.5.1.19 NAD+ competitive with respect to nicotinamide Mus musculus
3.5.1.19 sulfhydryl reagents
-
 Mus musculus
3.5.1.19 Triton X-100 0.1%, 24% inhibition Mus musculus
3.5.1.19 Urea 1 M, 20% inhibition Mus musculus
3.5.1.19 Zn2+
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.19 additional information
-
 additional information
-
 Mus musculus
3.5.1.19 0.006
-
 nicotinamide
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.19 Ca2+ activation Mus musculus
3.5.1.19 Mg2+ activation Mus musculus
3.5.1.19 Mn2+ activation Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.19 50000
-
 2 * 65000 + 2 * 50000, SDS-PAGE Mus musculus
3.5.1.19 65000
-
 2 * 65000 + 2 * 50000, SDS-PAGE Mus musculus
3.5.1.19 230000
-
 gel electrophoresis Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.19 nicotinamide + H2O Mus musculus enzyme is induced at high nicotinamide concentrations nicotinate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.19 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.19 glycoprotein contains 1.7% w/w mannose and 1.5% w/w N-acetylglucosamine Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.19
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.19 C-1300 cell C1300 neuroblastoma M1 clonal cell line Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.19 -20°C, 50% glycerol, stable for months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.19 nicotinamide + H2O
-
 Mus musculus nicotinate + NH3
-
 ?
3.5.1.19 nicotinamide + H2O enzyme is induced at high nicotinamide concentrations Mus musculus nicotinate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.19 tetramer 2 * 65000 + 2 * 50000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.19 37
-
-
 Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.19 55
-
 half-life: 2.5 min Mus musculus
3.5.1.19 65
-
 half-life: 1 min Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.19 6.8 7.5
-
 Mus musculus
3.5.1.19 7.2
-
-
 Mus musculus
3.5.1.19 7.5
-
-
 Mus musculus