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Literature summary extracted from

  • Kim, H.M.; Ryu, K.E.; Bae, K.S.; Rhee, Y.H.
    Purification and characterization of the extracellular medium-chain-length polyhydroxyalkanoate depolymerase from Pseudomonas sp. RY-1 (2000), J. Biosci. Bioeng., 89, 196-198.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 additional information not inhibited by dithioerythritol and phenylmethylsulfonyl fluoride Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 115000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas sp. utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O Pseudomonas sp. RY-1 utilization of bacterial PHA storage compounds poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas sp.
-
 RY-1
-
3.1.1.76 Pseudomonas sp. RY-1
-
 RY-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 poly[(R)-3-hydroxynonanoate]n + H2O
-
 Pseudomonas sp. ?
-
 ?
3.1.1.76 poly[(R)-3-hydroxynonanoate]n + H2O
-
 Pseudomonas sp. RY-1 ?
-
 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O
-
 Pseudomonas sp. poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas sp. poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O
-
 Pseudomonas sp. RY-1 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x: 1-5 ?
3.1.1.76 poly[(R)-3-hydroxyoctanoate]n + H2O utilization of bacterial PHA storage compounds Pseudomonas sp. RY-1 poly[(R)-3-hydroxyoctanoate]n-x + poly[(R)-3-hydroxyoctanoate]x x = 1-5 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 tetramer 4 identical subunits Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 35
-
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8.5
-
-
 Pseudomonas sp.