EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | cysteine | of partially purified preparation | Cavia porcellus |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.9 | crude water extract from liver acetone-powder unstable | Cavia porcellus |
1.1.1.9 | freezing/thawing inactivates, 8 cycles lead to 89% loss of activity and 11 cycles to complete inactivation | Cavia porcellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | D-xylulose | high concentration | Cavia porcellus | |
1.1.1.9 | additional information | no inhibition by EDTA or iodoacetate | Cavia porcellus | |
1.1.1.9 | PCMB | complete inhibition | Cavia porcellus | |
1.1.1.10 | iodoacetate | not inhibitory | Cavia porcellus | |
1.1.1.10 | additional information | not: EDTA | Cavia porcellus | |
1.1.1.10 | p-chloromercuribenzoate | - |
Cavia porcellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.9 | 0.66 | - |
D-xylulose | - |
Cavia porcellus | |
1.1.1.10 | 0.29 | - |
L-xylulose | - |
Cavia porcellus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.9 | cytoplasm | - |
Cavia porcellus | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | additional information | no evidence for metal requirement | Cavia porcellus |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.9 | Acetone | stable to precipitation with 50% v/v | Cavia porcellus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.9 | Cavia porcellus | - |
- |
- |
1.1.1.10 | Cavia porcellus | - |
guinea pig | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.9 | partial | Cavia porcellus |
1.1.1.10 | partial | Cavia porcellus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.9 | liver | - |
Cavia porcellus | - |
1.1.1.10 | liver | - |
Cavia porcellus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | additional information | - |
- |
Cavia porcellus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.9 | -10°C, acetone precipitate stable for weeks | Cavia porcellus |
1.1.1.9 | 0°C, partially purified stable for weeks | Cavia porcellus |
1.1.1.10 | frozen, ammonium sulfate precipitate, several days | Cavia porcellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.9 | D-ribitol + NAD+ | - |
Cavia porcellus | D-ribulose + NADH | - |
? | |
1.1.1.9 | D-sorbitol + NAD+ | - |
Cavia porcellus | D-fructose + NADH + H+ | - |
? | |
1.1.1.9 | D-xylulose + NADH + H+ | - |
Cavia porcellus | xylitol + NAD+ | - |
r | |
1.1.1.9 | L-arabitol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
1.1.1.9 | L-erythrulose + NADH | reduction at the same rate as D-xylulose | Cavia porcellus | erythritol + NAD+ | - |
? | |
1.1.1.9 | L-iditol + NAD+ | - |
Cavia porcellus | ? | - |
? | |
1.1.1.9 | additional information | no reduction of D-ribose and D-galacturonic acid | Cavia porcellus | ? | - |
? | |
1.1.1.9 | additional information | no oxidation of threitol, xylitol, sorbitol, D-iditol | Cavia porcellus | ? | - |
? | |
1.1.1.9 | additional information | no reduction of D-ribulose | Cavia porcellus | ? | - |
? | |
1.1.1.9 | additional information | no reduction of D/L-xylose | Cavia porcellus | ? | - |
? | |
1.1.1.9 | xylitol + NAD+ | - |
Cavia porcellus | D-xylulose + NADH + H+ | - |
r | |
1.1.1.10 | xylitol + NADP+ | - |
Cavia porcellus | L-xylulose + NADPH + H+ | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 7 | - |
substrate reduction | Cavia porcellus |
1.1.1.10 | 7 | - |
- |
Cavia porcellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.9 | 4 | 8 | about 75% of maximal reduction activity at pH 4.0 and 8.0 | Cavia porcellus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.9 | additional information | no activity with NADPH | Cavia porcellus | |
1.1.1.9 | additional information | no activity with NADP+ | Cavia porcellus | |
1.1.1.9 | additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Cavia porcellus | |
1.1.1.9 | NAD+ | - |
Cavia porcellus | |
1.1.1.9 | NADH | - |
Cavia porcellus | |
1.1.1.10 | additional information | - |
Cavia porcellus |