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Literature summary extracted from
- Allantoin racemase: a new enzyme from Pseudomonas species (1975), Biochim. Biophys. Acta, 391, 240-248.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.3 | dithiothreitol | preincubation for 30 min at 30 C stimulates | Comamonas testosteroni |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.1.99.3 | repeated freezing and thawing inactivates | Comamonas testosteroni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.99.3 | 1,10-phenanthroline | - |
Comamonas testosteroni | |
| 5.1.99.3 | 5-Aminohydantoin | - |
Comamonas testosteroni | |
| 5.1.99.3 | Co2+ | 0.8 mM | Comamonas testosteroni | |
| 5.1.99.3 | hydantoin | - |
Comamonas testosteroni | |
| 5.1.99.3 | Hydantoin of N-carbamoyl-L-methionine | - |
Comamonas testosteroni | |
| 5.1.99.3 | iodoacetate | - |
Comamonas testosteroni | |
| 5.1.99.3 | Mn2+ | 0.8 mM | Comamonas testosteroni | |
| 5.1.99.3 | NH2OH | - |
Comamonas testosteroni | |
| 5.1.99.3 | Zn2+ | 0.8 mM | Comamonas testosteroni | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.99.3 | Comamonas testosteroni | - |
- |
- |
| 5.1.99.3 | Pseudomonas fluorescens | - |
- |
- |
| 5.1.99.3 | Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.99.3 | - |
Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | additional information | - |
- |
Comamonas testosteroni |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.1.99.3 | 4°C, stable for several weeks | Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.99.3 | (S)(+)-Allantoin | - |
Pseudomonas fluorescens | (R)(-)-Allantoin | - |
? | |
| 5.1.99.3 | (S)(+)-Allantoin | - |
Pseudomonas putida | (R)(-)-Allantoin | - |
? | |
| 5.1.99.3 | (S)(+)-Allantoin | r | Comamonas testosteroni | (R)(-)-Allantoin | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | 50 | - |
- |
Comamonas testosteroni |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | 20 | 60 | 20°C: about 35% of maximal activity, 30°C: 67% of maximal activity, 60°C: about 85% of maximal activity | Comamonas testosteroni |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | 70 | - |
15 min, pH 6.5-7, stable up to | Comamonas testosteroni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | 8 | 8.2 | - |
Comamonas testosteroni |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.99.3 | 6.5 | 7 | up to 70°C, 15 min, stable | Comamonas testosteroni |
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Release 2023.1 (January 2023)
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