EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.99.1 | analysis | a simple direct assay for DL-methylmalonyl-coenzyme A racemase which is based on the fact that the proton on C-2 of methylmalonyl-CoA is replaced by a proton in the medium during racemization | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.99.1 | acetyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | butyryl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | Cd2+ | - |
Rattus norvegicus | |
5.1.99.1 | CoA | - |
Rattus norvegicus | |
5.1.99.1 | Cu2+ | - |
Rattus norvegicus | |
5.1.99.1 | EDTA | reactivation by Co2+ | Rattus norvegicus | |
5.1.99.1 | glutaryl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | heptanoyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | malonyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | octanoyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | propionyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | succinyl-CoA | - |
Rattus norvegicus | |
5.1.99.1 | Zn2+ | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.99.1 | 0.1 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.1.99.1 | Co2+ | most potent activator, Km: 0.0002 mM, binds 2 mol Co2+ per mol of racemase | Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.99.1 | 16000 | - |
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
5.1.99.1 | 32000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.99.1 | additional information | Rattus norvegicus | enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.99.1 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.99.1 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.1.99.1 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.99.1 | 8400 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
5.1.99.1 | -20°C, stable for at least 3 months | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.99.1 | (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Rattus norvegicus | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
5.1.99.1 | DL-methylmalonyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
5.1.99.1 | additional information | enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.99.1 | dimer | 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.99.1 | 4170 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.99.1 | 7 | - |
- |
Rattus norvegicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.1.99.1 | 5.5 | 8.5 | 50% of maximal activity at pH 5.5 and 8.5 | Rattus norvegicus |