Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stabler, S.P.; Marcell, P.D.; Allen, R.H.
    Isolation and characterization of DL-methylmalonyl-coenzyme A racemase from rat liver (1985), Arch. Biochem. Biophys., 241, 252-264.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.99.1 analysis a simple direct assay for DL-methylmalonyl-coenzyme A racemase which is based on the fact that the proton on C-2 of methylmalonyl-CoA is replaced by a proton in the medium during racemization Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.99.1 acetyl-CoA
-
Rattus norvegicus
5.1.99.1 butyryl-CoA
-
Rattus norvegicus
5.1.99.1 Cd2+
-
Rattus norvegicus
5.1.99.1 CoA
-
Rattus norvegicus
5.1.99.1 Cu2+
-
Rattus norvegicus
5.1.99.1 EDTA reactivation by Co2+ Rattus norvegicus
5.1.99.1 glutaryl-CoA
-
Rattus norvegicus
5.1.99.1 heptanoyl-CoA
-
Rattus norvegicus
5.1.99.1 malonyl-CoA
-
Rattus norvegicus
5.1.99.1 octanoyl-CoA
-
Rattus norvegicus
5.1.99.1 propionyl-CoA
-
Rattus norvegicus
5.1.99.1 succinyl-CoA
-
Rattus norvegicus
5.1.99.1 Zn2+
-
Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.99.1 0.1
-
DL-methylmalonyl-CoA
-
Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.99.1 Co2+ most potent activator, Km: 0.0002 mM, binds 2 mol Co2+ per mol of racemase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.99.1 16000
-
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol Rattus norvegicus
5.1.99.1 32000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.99.1 additional information Rattus norvegicus enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.99.1
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.99.1 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.99.1 8400
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
5.1.99.1 -20°C, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.1 (R)-2-Methyl-3-oxopropanoyl-CoA
-
Rattus norvegicus (S)-2-Methyl-3-oxopropanoyl-CoA
-
?
5.1.99.1 DL-methylmalonyl-CoA
-
Rattus norvegicus ?
-
?
5.1.99.1 additional information enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.99.1 dimer 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.99.1 4170
-
DL-methylmalonyl-CoA
-
Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.99.1 7
-
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.99.1 5.5 8.5 50% of maximal activity at pH 5.5 and 8.5 Rattus norvegicus