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Literature summary extracted from

  • Hashimoto, J.; Uchida, T.; Egami, F.
    Purification of ribonuclease U 1 and some properties of ribonucleases U 1 and N 1 (1971), J. Biochem., 70, 903-911.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.24 iodoacetate Ribunuclease U1 Ustilago sphaerogena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.24 0.069
-
 GpC RNase N1 Ustilago sphaerogena
4.6.1.24 0.238
-
 GpC RNase U1 Ustilago sphaerogena
4.6.1.24 0.435
-
 GpC RNase T1 Ustilago sphaerogena

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.24 Ustilago sphaerogena
-
 similar enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.24 similar enzyme Ustilago sphaerogena

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.24 culture medium
-
 Ustilago sphaerogena
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.24 additional information
-
-
 Ustilago sphaerogena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.24 GpC + H2O
-
 Ustilago sphaerogena guanosine 3'-phosphate + cytidine
-
 ?
4.6.1.24 guanosine 2',3'-cyclic phosphate + H2O Ustilago sphaerogena, RNase U1 Ustilago sphaerogena guanosine-3'-phosphate
-
 ?
4.6.1.24 RNA + H2O
-
 Ustilago sphaerogena Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.24 7
-
 RNase N1 Ustilago sphaerogena
4.6.1.24 8
-
 RNase U1 Ustilago sphaerogena