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Literature summary extracted from

  • Ito, H.; Hagiwara, M.; Takahashi, K.; Ichikizaki, I.
    The structure and function of ribonuclease T1 XXIV. Preparation and properties of a stable water-insoluble polyacrylamide derivative of ribonuclease T1 (1977), J. Biochem., 82, 877-883.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.6.1.24 Immobilized enzyme, heat- and pH-stability Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.24 10000
-
Neurospora crassa, RNase N4, gel filtration, SDS-PAGE Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.24 Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.24 RNA + H2O
-
Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.24 43
-
immobilized enzyme Aspergillus oryzae
4.6.1.24 45
-
native enzyme Aspergillus oryzae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.6.1.24 20 55 20°C: 18% of activity maximum, 55°C: 15% of activity maximum, inactive at 60°C, native enzyme Aspergillus oryzae
4.6.1.24 38 80 38°C: 55% of activity maximum, 80°C: 40% of activity maximum, immobilized enzyme Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.24 100
-
30 min, immobilized enzyme: 5% loss of activity, native enzyme: about 60% loss of activity Aspergillus oryzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.24 7.3
-
immobilized enzyme Aspergillus oryzae
4.6.1.24 7.8
-
native enzyme Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.6.1.24 4 9 4: 10% (native), 20% (immobilized) of activity maximum, 9: 32% (native), 25% (immobilized) of activity maximum, 10: immobilized enzyme, some activity, native enzyme, inactive Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.6.1.24 4 10 37°C, 33 h, immobilized enzyme, highest stability at pH 7, at pH 1.0 and 10.0: about 60% of activity maximum Aspergillus oryzae