EC Number | General Stability | Organism |
---|---|---|
4.6.1.24 | Immobilized enzyme, heat- and pH-stability | Aspergillus oryzae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 10000 | - |
Neurospora crassa, RNase N4, gel filtration, SDS-PAGE | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.6.1.24 | Aspergillus oryzae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.6.1.24 | RNA + H2O | - |
Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 43 | - |
immobilized enzyme | Aspergillus oryzae |
4.6.1.24 | 45 | - |
native enzyme | Aspergillus oryzae |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 20 | 55 | 20°C: 18% of activity maximum, 55°C: 15% of activity maximum, inactive at 60°C, native enzyme | Aspergillus oryzae |
4.6.1.24 | 38 | 80 | 38°C: 55% of activity maximum, 80°C: 40% of activity maximum, immobilized enzyme | Aspergillus oryzae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 100 | - |
30 min, immobilized enzyme: 5% loss of activity, native enzyme: about 60% loss of activity | Aspergillus oryzae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 7.3 | - |
immobilized enzyme | Aspergillus oryzae |
4.6.1.24 | 7.8 | - |
native enzyme | Aspergillus oryzae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 4 | 9 | 4: 10% (native), 20% (immobilized) of activity maximum, 9: 32% (native), 25% (immobilized) of activity maximum, 10: immobilized enzyme, some activity, native enzyme, inactive | Aspergillus oryzae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.6.1.24 | 4 | 10 | 37°C, 33 h, immobilized enzyme, highest stability at pH 7, at pH 1.0 and 10.0: about 60% of activity maximum | Aspergillus oryzae |