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Literature summary extracted from

  • Davies, H.M.; Anderson, L.; Fan, C.; Hawkins, D.J.
    Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica (1991), Arch. Biochem. Biophys., 290, 37-45.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.21 CHAPS stimulation Umbellularia californica
3.1.2.21 cholate slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v Umbellularia californica
3.1.2.21 octylglucoside stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v Umbellularia californica
3.1.2.21 Triton X-100 stimulation Umbellularia californica
3.1.2.21 Zwittergent 3-8 stimulation Umbellularia californica

General Stability

EC Number General Stability Organism
3.1.2.21 stable to repeated thawings Umbellularia californica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.21 Ca2+ 1 mM, 50% inhibition Umbellularia californica
3.1.2.21 cholate slight stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v Umbellularia californica
3.1.2.21 Cu2+ 1 mM, 100% inhibition Umbellularia californica
3.1.2.21 diethyldicarbonate 1 mM, 97% inhibition Umbellularia californica
3.1.2.21 Mg2+ 1 mM, 50% inhibition Umbellularia californica
3.1.2.21 Mn2+ 1 mM, 50% inhibition Umbellularia californica
3.1.2.21 octylglucoside stimulation at 0.1 and 0.01% w/v. Strong inhibition at 1% w/v Umbellularia californica
3.1.2.21 Zn2+ 1 mM, 100% inhibition Umbellularia californica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.21 0.002 0.003 dodecanoyl-[acyl-carrier protein]
-
 Umbellularia californica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.21 NaCl optimal concentration: 20-40 mM Umbellularia californica
3.1.2.21 phosphate optimal concentration: 15-20 mM Umbellularia californica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.21 34000
-
 1 * 34000, SDS-PAGE Umbellularia californica
3.1.2.21 42000
-
 gel filtration Umbellularia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.21 dodecanoyl-[acyl-carrier protein] + H2O Umbellularia californica enzyme plays a major role in medium-chain fatty acid production [acyl-carrier protein] + dodecanoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Umbellularia californica
-
-
-
3.1.2.21 Umbellularia californica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.21
-
 Umbellularia californica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.14 cotyledon
-
 Umbellularia californica
-
3.1.2.21 cotyledon immature Umbellularia californica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.21 5.17
-
-
 Umbellularia californica

Storage Stability

EC Number Storage Stability Organism
3.1.2.21 -70°C, stable Umbellularia californica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 lauroyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica lauric acid + acyl-carrier protein
-
 ?
3.1.2.21 dodecanoyl-[acyl-carrier protein] + H2O
-
 Umbellularia californica [acyl-carrier protein] + dodecanoate
-
 ?
3.1.2.21 dodecanoyl-[acyl-carrier protein] + H2O enzyme plays a major role in medium-chain fatty acid production Umbellularia californica [acyl-carrier protein] + dodecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.21 monomer 1 * 34000, SDS-PAGE Umbellularia californica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.21 1
-
 Mg2+
-
 Umbellularia californica
3.1.2.21 1
-
 Mn2+
-
 Umbellularia californica
3.1.2.21 1
-
 Ca2+
-
 Umbellularia californica