Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kinoshita, S.; Kadota, K.; Taguchi, H.
    Purification and properties of aldose 1-epimerase from Aspergillus niger (1981), Biochim. Biophys. Acta, 662, 285-290.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.3 analysis rapid determination of glucose by aldose 1-epimerase Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.3 50
-
 alpha-D-glucose
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.3 130000
-
 2 * 130000, SDS-PAGE Aspergillus niger
5.1.3.3 260000
-
 gel filtration Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.3 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.3
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.3 4.6
-
-
 Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.3 alpha-D-galactose 105% of the activity relative to alpha-D-glucose Aspergillus niger beta-D-galactose
-
 ?
5.1.3.3 alpha-D-glucose
-
 Aspergillus niger beta-D-glucose
-
 ?
5.1.3.3 alpha-D-xylose 31% of the activity relative to alpha-D-glucose Aspergillus niger beta-D-xylose
-
 ?
5.1.3.3 alpha-L-arabinose beta-L-arabinose is epimerized at 108% of the activity relative to alpha-D-glucose Aspergillus niger beta-L-arabinose
-
 ?
5.1.3.3 beta-D-Cellobiose 15% of the activity relative to alpha-D-glucose Aspergillus niger ?
-
 ?
5.1.3.3 beta-D-Fructose 135% of the activity relative to alpha-D-glucose Aspergillus niger alpha-D-Fructose
-
 ?
5.1.3.3 beta-Lactose 10% of the activity relative to alpha-D-glucose Aspergillus niger alpha-Lactose
-
 ?
5.1.3.3 beta-Maltose 13% of the activity relative to alpha-D-glucose Aspergillus niger alpha-Maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.3 dimer 2 * 130000, SDS-PAGE Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.3 55
-
 30 min, stable below Aspergillus niger
5.1.3.3 60
-
 30 min, 5% loss of activity Aspergillus niger
5.1.3.3 65
-
 30 min, 25% loss of activity Aspergillus niger
5.1.3.3 75
-
 half-life: 6.9 min Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.3 2600
-
 alpha-D-glucose
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.3 5 7
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.3 2
-
 20°C, 24 h, complete loss of activity Aspergillus niger
5.1.3.3 3
-
 20°C, 24 h, 50% loss of activity Aspergillus niger
5.1.3.3 5.5 9 20°C, 24 h, no inactivation Aspergillus niger
5.1.3.3 10.5
-
 20°C, 24 h, 66% loss of activity Aspergillus niger
5.1.3.3 11
-
 20°C, 24 h, complete loss of activity Aspergillus niger